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1.发明申请Induction of Pancreatic Stem Cells by Transient Overexpression of Reprogramming Factors and PDX1 Selection 审中-公开通过重新编程因子和PDX1选择的瞬时过表达诱导胰腺干细胞公开(公告)号：US20120135015A1
公开(公告)日：2012-05-31
申请号：US13236367
申请日：2011-09-19
CPC分类号： C12N5/0676 , A61K35/12 , A61K35/39 , C12N2501/105 , C12N2501/119 , C12N2501/12 , C12N2501/16 , C12N2501/335 , C12N2501/385 , C12N2501/41 , C12N2501/415 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2502/1323 , C12N2506/22 , C12N2510/00
摘要： Methods for generating pancreatic stem cells from a pancreatic tissue of 24-week old mice by transient overexpression of reprogramming factors combined with Pdx1 selection is described herein. The generated cells were designated as iPaS (induced pancreatic stem) cells and exhibit the same morphology as the pancreatic stem cells previously established from young donors without genetic manipulation and express genetic markers of endoderm and pancreatic progenitors. Transplantation of the iPaS cells into nude mice resulted in no teratoma formation. Moreover, iPaS cells were able to differentiate into insulin-producing cells more efficiently than ES cells. In addition, the technology of transient overexpression of reprogramming factors and tissue-specific selection of the present invention may also be useful for the generation of other tissue-specific stem cells.
摘要翻译： 本文描述了通过与Pdx1选择结合的重编程因子的瞬时过表达从24周龄小鼠的胰腺组织产生胰腺干细胞的方法。 所产生的细胞被命名为iPaS（诱导的胰腺干细胞），并表现出与先前由年轻供体建立的胰腺干细胞相同的形态，而没有遗传操作，并表达内胚层和胰腺祖细胞的遗传标记。 将iPaS细胞移植到裸鼠中导致没有畸胎瘤形成。 此外，iPaS细胞能够比ES细胞更有效地分化成胰岛素生成细胞。 此外，本发明的重编程因子的瞬时过表达和组织特异性选择的技术也可用于产生其他组织特异性干细胞。
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2.发明授权公开(公告)号：US08637494B2
公开(公告)日：2014-01-28
申请号：US13160334
申请日：2011-06-14
CPC分类号： A61K31/58
摘要： Method and compositions for reducing the inflammation after cytokine exposure of an islet cell transplant without affecting the viability and potency are disclosed herein. The present invention describes a composition comprising Withaferin A (WA), a steroidal lactone derived from Withania somnifera to effectively block NF-kB activation in beta cells, minimize cytokine-induced cell death and improve survival of transplanted islets. The method of the present invention involves identifying a subject having islet cells in need of treatment; and providing a effective amount of a Withaferin A composition disposed in a pharmaceutically acceptable carrier in an amount sufficient to reduce the inflammation after cytokine exposure without affecting the viability and potency of the islet cell population.
摘要翻译： 本文公开了用于减少细胞因子暴露于胰岛细胞移植而不影响生存力和效力的炎症的方法和组合物。 本发明描述了一种组合物，其包含嗜铬蛋白A（WA），来自嗜睡细菌的甾体内酯，以有效阻断β细胞中的NF-κB活化，使细胞因子诱导的细胞死亡最小化并提高移植胰岛的存活。 本发明的方法涉及鉴定具有需要治疗的胰岛细胞的受试者; 并且以足以减少细胞因子暴露后的炎症而不影响胰岛细胞群体的活力和潜力的量，提供有效量的药物可接受的载体中配置的伴有咖啡因的组合物。
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公开(公告)号：US20110305719A1
公开(公告)日：2011-12-15
申请号：US13160334
申请日：2011-06-14
CPC分类号： A61K31/58
摘要： Method and compositions for reducing the inflammation after cytokine exposure of an islet cell transplant without affecting the viability and potency are disclosed herein. The present invention describes a composition comprising Withaferin A (WA), a steroidal lactone derived from Withania somnifera to effectively block NF-kB activation in beta cells, minimize cytokine-induced cell death and improve survival of transplanted islets. The method of the present invention involves identifying a subject having islet cells in need of treatment; and providing a effective amount of a Withaferin A composition disposed in a pharmaceutically acceptable carrier in an amount sufficient to reduce the inflammation after cytokine exposure without affecting the viability and potency of the islet cell population.
摘要翻译： 本文公开了用于减少细胞因子暴露于胰岛细胞移植而不影响生存力和效力的炎症的方法和组合物。 本发明描述了一种组合物，其包含嗜铬蛋白A（WA），来自嗜睡细菌的甾体内酯，以有效阻断β细胞中的NF-κB活化，使细胞因子诱导的细胞死亡最小化并提高移植胰岛的存活。 本发明的方法涉及鉴定具有需要治疗的胰岛细胞的受试者; 并且以足以减少细胞因子暴露后的炎症而不影响胰岛细胞群体的活力和潜力的量，提供有效量的药物可接受的载体中配置的伴有咖啡因的组合物。
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