公开(公告)号：US06539244B2

公开(公告)日：2003-03-25

申请号：US09740817

申请日：2000-12-21

申请人： Hirokazu Kato ,  Jun Otomo ,  Sayuri Nomura ,  Tomoko Takeshita ,  Sakae Saito ,  Shokichi Matsunami ,  Noboru Moriya

发明人： Hirokazu Kato ,  Jun Otomo ,  Sayuri Nomura ,  Tomoko Takeshita ,  Sakae Saito ,  Shokichi Matsunami ,  Noboru Moriya

IPC分类号： A61B505

CPC分类号： G01N33/48728

摘要： An automatic electrophysiological measuring apparatus to automatically penetrate a glass electrode(s) into the membrane of a Xenopus Oocyte to hold the membrane potential and to automatically measure reactions to the administration of a medicine is to be provided. A glass electrode 103 in the air is moved toward a Xenopus Oocyte 601 held in a cell. Changes in the potential states of the glass electrodes are picked up by a control computer to distinguish the relative positions of the glass electrode 102 and the Xenopus Oocyte 601, and the sequence of electrophysiological measurement so as to penetrate the glass electrode 102 into the Xenopus Oocyte 601, hold the membrane potential and automatically administer the medicine.

摘要翻译： 提供一种自动电生理测量装置，用于将玻璃电极自动穿透到非洲爪蟾卵母细胞的膜中以保持膜电位并且自动测量对药物施用的反应。 空气中的玻璃电极103朝向保持在电池中的爪蟾卵母细胞601移动。 由控制计算机拾取玻璃电极的电位状态的变化，以区分玻璃电极102和非洲爪蟾卵母细胞601的相对位置，以及电生理测量序列，以便穿透玻璃电极102进入非洲爪蟾卵母细胞 601，保持膜电位并自动管理药物。

公开(公告)号：US06470201B2

公开(公告)日：2002-10-22

申请号：US09799119

申请日：2001-03-06

申请人： Hirokazu Kato ,  Jun Otomo ,  Sayuri Nomura ,  Tomoko Takeshita ,  Sakae Saito ,  Shokichi Matsunami ,  Noboru Moriya

发明人： Hirokazu Kato ,  Jun Otomo ,  Sayuri Nomura ,  Tomoko Takeshita ,  Sakae Saito ,  Shokichi Matsunami ,  Noboru Moriya

IPC分类号： A61B505

CPC分类号： G01N33/48728

摘要： An automatic electrophysiological measuring apparatus to automatically penetrate a glass electrode(s) into the membrane of a Xenopus Oocyte to hold the membrane potential and to automatically measure reactions to the administration of a medicine is to be provided. A glass electrode 103 in the air is moved toward a Xenopus Oocyte 601 held in a cell. Changes in the potential states of the glass electrodes are picked up by a control computer to distinguish the relative positions of the glass electrode 102 and the Xenopus Oocyte 601 and the sequence of electrophysiological measurement so as to penetrate the glass electrode 102 into the Xenopus Oocyte 601, hold the membrane potential and automatically administer the medicine.

摘要翻译： 本发明提供一种自动电生理测量装置，用于将玻璃电极自动穿透到非洲爪蟾卵母细胞膜以保持膜电位并自动测量药物施用反应。空气中的玻璃电极103 移动到一个牢房中的非洲爪蟾卵母细胞601。 通过控制计算机拾取玻璃电极的电位状态的变化，以区分玻璃电极102和非洲爪蟾卵母细胞601的相对位置和电生理测量序列，以便穿透玻璃电极102进入非洲爪蟾卵母细胞601 ，保持膜电位并自动管理药物。

公开(公告)号：US06593129B1

公开(公告)日：2003-07-15

申请号：US09666530

申请日：2000-09-20

申请人： Tomoko Takeshita ,  Jun Otomo ,  Sayuri Nomura ,  Shokichi Matsunami ,  Noboru Moriya ,  Sakae Saito

发明人： Tomoko Takeshita ,  Jun Otomo ,  Sayuri Nomura ,  Shokichi Matsunami ,  Noboru Moriya ,  Sakae Saito

IPC分类号： C12M100

CPC分类号： C12M23/50 ,  C12M35/00

摘要： An apparatus for microinjection of samples into amphibian oocytes, comprising a tray for holding a plurality of the amphibian oocytes, an injection needle for injecting a sample into the said amphibian oocytes, a driving means for moving a relative position between the said tray and the said injection needle and a controlling means for controlling the said movement by imputing a depth of the said injection needle for the said tray or the said amphibian oocytes in the injection of the sample, and injecting the sample into the said amphibian oocytes at the said depth. According to the present invention, the sample can be injected into the amphibian oocyte with constant depth.precisely and quality of oocyte or a positional site of needle injection can be recorded as the information.

摘要翻译： 一种用于将样品显微注射到两栖动物卵母细胞中的装置，包括用于保持多个两栖动物卵母细胞的托盘，用于将样品注入所述两栖动物卵母细胞的注射针;驱动装置，用于使所述托盘和所述 注射针和控制装置，用于通过在注射样品时插入所述托盘或所述两栖动物卵母细胞的所述注射针的深度来控制所述运动，以及在所述深度将样品注入所述两栖动物卵母细胞。 根据本发明，可以将样品以恒定的深度精确地注入到两栖动物卵母细胞中，并且可以记录卵母细胞的质量或针注射的位置位置作为信息。

公开(公告)号：US06803207B2

公开(公告)日：2004-10-12

申请号：US10145099

申请日：2002-05-15

申请人： Tomoko Takeshita ,  Jun Otomo ,  Shokichi Matsunami

发明人： Tomoko Takeshita ,  Jun Otomo ,  Shokichi Matsunami

IPC分类号： C12Q102

CPC分类号： C12M21/06 ,  C12M41/46 ,  C12M47/04

摘要： A method or an apparatus for selecting oocytes or eggs based on at least one objective criterion, such as membrane potential, comprises a step or means for selecting a plurality of oocytes or eggs having a certain size with a filter or the like, and a step or means for measuring a membrane potential of each of the oocytes or eggs thereby sorting out those with a membrane potential within a specified range. The selected oocytes or eggs are sold or transferred together with the measurement information or an injected sample of interest.

摘要翻译： 基于至少一个客观标准（例如膜电位）来选择卵母细胞或卵的方法或装置包括用过滤器等选择具有一定尺寸的多个卵母细胞或卵的步骤或装置，以及步骤 或用于测量每个卵母细胞或卵的膜电位的方法，从而将具有膜电位的那些分选到指定范围内。 所选择的卵母细胞或蛋与测量信息或注射的感兴趣样品一起出售或转移。

公开(公告)号：US06468784B1

公开(公告)日：2002-10-22

申请号：US10022734

申请日：2001-12-20

申请人： Tomoko Takeshita ,  Jun Otomo ,  Shokichi Matsunami

发明人： Tomoko Takeshita ,  Jun Otomo ,  Shokichi Matsunami

IPC分类号： C12M134

CPC分类号： C12M21/06 ,  C12M41/46 ,  C12M47/04

摘要： A method or an apparatus for selecting oocytes or eggs based on at least one objective criterion, such as membrane potential, comprises a step or means for selecting a plurality of oocytes or eggs having a certain size with a filter or the like, and a step or means for measuring a membrane potential of each of the oocytes or eggs thereby sorting out those with a membrane potential within a specified range. The selected oocytes or eggs are sold or transferred together with the measurement information or an injected sample of interest.

摘要翻译： 基于至少一个客观标准（例如膜电位）来选择卵母细胞或卵的方法或装置包括用过滤器等选择具有一定尺寸的多个卵母细胞或卵的步骤或装置，以及步骤 或用于测量每个卵母细胞或卵的膜电位的方法，从而将具有膜电位的那些分选到指定范围内。 所选择的卵母细胞或蛋与测量信息或注射的感兴趣样品一起出售或转移。

公开(公告)号：US06338960B2

公开(公告)日：2002-01-15

申请号：US09788481

申请日：2001-02-21

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12M300

CPC分类号： G01F1/00 ,  G01N2333/726 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/826 ,  Y10T436/108331

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.

公开(公告)号：US06337178B2

公开(公告)日：2002-01-08

申请号：US09843866

申请日：2001-04-30

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12M300

CPC分类号： G01F1/00 ,  G01N2333/726 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/826 ,  Y10T436/108331

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.

摘要翻译： 组胺可以通过以下步骤进行定量测定。 首先，表达组胺受体的卵母细胞被保持在形成于血管底部的凹陷中。 然后，将第一和第二电极插入卵母细胞。 随后，通过使用第一电极通过使用用于钳位卵母细胞的膜电位的电路驱动通过第二电极的电流来将该膜电位稳定在预定水平来测量卵母细胞的膜电位。 然后将样品输入到具有固定在其内表面上的抗原与一些缓冲溶液的细小反应管中，以促进组胺释放反应。 将包含在细反应管中释放的组胺的溶液转移到容器中以与容器中的卵母细胞接触。 最后，检测由与溶液接触引起的卵母细胞的电响应，并确定微反应管中组胺释放反应中释放的组胺浓度。 整个血液或肥大细胞悬浮液可以用作样品而不进行预处理。

公开(公告)号：US06420168B1

公开(公告)日：2002-07-16

申请号：US09984614

申请日：2001-10-30

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12M300

CPC分类号： G01F1/00 ,  G01N2333/726 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/826 ,  Y10T436/108331

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.

公开(公告)号：US06277559B1

公开(公告)日：2001-08-21

申请号：US09788361

申请日：2001-02-21

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12Q100

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.

公开(公告)号：US06268121B1

公开(公告)日：2001-07-31

申请号：US09604512

申请日：2000-06-27

申请人： Tomoko Takeshita ,  Jun Otomo

发明人： Tomoko Takeshita ,  Jun Otomo

IPC分类号： C12Q100

CPC分类号： G01F1/00 ,  G01N2333/726 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/826 ,  Y10T436/108331

摘要： Histamine may be quantitatively measured by performing the following steps. First, an oocyte that expresses histamine receptors is held in a recess formed at the bottom of a vessel. Then, first and second electrodes are inserted into the oocyte. Subsequently, the membrane potential of the oocyte is measured by using the first electrode to stabilize this membrane potential at a predetermined level by driving a current through the second electrode using circuitry for clamping the membrane potential of the oocyte. A sample is then infused into a fine reacting tube having an antigen immobilized on its inner surface together with some buffer solution to promote a histamine releasing reaction. The solution containing histamines that is released in the fine reacting tube is transferred to the vessel to make contact with the oocyte in the vessel. Finally, an electric response of the oocyte caused by the contact with the solution is detected and the concentration of the histamine released in the histamine releasing reaction in the fine reacting tube is determined. The entire blood or mast cell suspension may be used as a sample without pretreatment.