公开(公告)号：US07927853B2

公开(公告)日：2011-04-19

申请号：US11473272

申请日：2006-06-23

申请人： Hirokazu Nishida ,  Yoshizumi Ishino ,  Kosuke Morikawa

发明人： Hirokazu Nishida ,  Yoshizumi Ishino ,  Kosuke Morikawa

IPC分类号： C12N9/10 ,  C12N9/00

CPC分类号： C12N9/93

摘要： It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.

摘要翻译： 旨在获得提高与DNA的结合能力和反应性的DNA连接酶，特别是耐热DNA连接酶，其与DNA的结合能力和反应性提高。 本发明提供了通过部分或完全缺失DNA连接酶的C末端螺旋部分获得的与DNA的结合能力和反应性提高的DNA连接酶突变体。 特别地，突变体来自耐热细菌。

公开(公告)号：US20070037190A1

公开(公告)日：2007-02-15

申请号：US11473272

申请日：2006-06-23

申请人： Hirokazu Nishida ,  Yoshizumi Ishino ,  Kosuke Morikawa

发明人： Hirokazu Nishida ,  Yoshizumi Ishino ,  Kosuke Morikawa

IPC分类号： C12Q1/68 ,  C12N9/22 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/93

摘要： It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.

摘要翻译： 旨在获得提高与DNA的结合能力和反应性的DNA连接酶，特别是耐热DNA连接酶，其与DNA的结合能力和反应性提高。 本发明提供了通过部分或完全缺失DNA连接酶的C末端螺旋部分获得的与DNA的结合能力和反应性提高的DNA连接酶突变体。 特别地，突变体来自耐热细菌。