公开(公告)号：US5094947A

公开(公告)日：1992-03-10

申请号：US758481

申请日：1991-09-09

申请人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Turutani ,  Hayato Ishihara

发明人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Turutani ,  Hayato Ishihara

IPC分类号： C12P19/02 ,  C12R1/01 ,  C12R1/07 ,  C12R1/19

CPC分类号： C12P19/02

摘要： A process for producing fructose-1,6-diphosphate comprising the steps of: (a) enzymatically converting adenosine 5'-diphosphate to adenosine 5'-triphosphate using an acetate kinase-containing microorganism or an extract of the microorganism and phosphate donor; and (b) enzymatically converting a substrate capable of being converted to glucose or fructose to fructose-1,6-diphosphate using the adenosine 5'-triphosphate resulting from step (a) and the acetate kinase-containing microorganism or the extract of the microorganism.

公开(公告)号：US5434255A

公开(公告)日：1995-07-18

申请号：US37395

申请日：1993-03-26

申请人： Tatsuo Katayama ,  Hayato Ishihara ,  Kenji Okada ,  Masaaki Onda ,  Hiroshi Nakajima

发明人： Tatsuo Katayama ,  Hayato Ishihara ,  Kenji Okada ,  Masaaki Onda ,  Hiroshi Nakajima

IPC分类号： B01D15/08 ,  B01D61/02 ,  B01D61/44 ,  B01J41/20 ,  C07H1/06 ,  C07H11/04 ,  C07H13/00

CPC分类号： C07H11/04

摘要： A process for the purification of fructose 1,6-diphosphate which comprises subjecting a fructose 1,6-diphosphate-containing solution to anion exchange column chromatography to separate fructose 1,6-diphosphate therefrom, and subsequently subjecting the resulting FDP fraction to a desalting treatment, so as to provide a highly purified FDP preparation useful as a pharmaceutical drug and the like in a high yield.

摘要翻译： 1,6-二磷酸果糖的纯化方法，该方法包括将含有1,6-二磷酸的果糖溶液进行阴离子交换柱层析，从其中分离出果糖1,6-二磷酸酯，然后将得到的FDP馏分进行脱盐 进行处理，从而以高产率提供可用作药物等的高度纯化的FDP制剂。