公开(公告)号：US11732245B2

公开(公告)日：2023-08-22

申请号：US17379359

申请日：2021-07-19

Applicant: The Trustees of the University of Pennsylvania

Inventor： Martin Lock ,  Mauricio Alvira

IPC: C12N7/02 ,  C12N15/86 ,  B01D15/38 ,  B01J20/281 ,  C12N15/864 ,  G01N21/33 ,  B01J41/05 ,  B01D15/16 ,  B01D15/36 ,  B01J41/20

CPC classification number: C12N7/02 ,  B01D15/3804 ,  B01J20/281 ,  C12N15/86 ,  C12N15/8645 ,  G01N21/33 ,  B01D15/166 ,  B01D15/363 ,  B01J41/05 ,  B01J41/20 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

公开(公告)号：US11628381B2

公开(公告)日：2023-04-18

申请号：US14374777

申请日：2013-09-16

Applicant: W. R. Grace & Co.-Conn.

Inventor： Feng Gu ,  Ning Mu

IPC: B01J20/28 ,  G01N30/52 ,  B01J20/32 ,  B01D15/36 ,  B01J39/26 ,  B01J41/20 ,  B01D15/26 ,  B01J20/289

Abstract: Chromatography devices contain chromatography media and methods of making and methods of using chromatography devices. Chromatography devices enable a more efficient, productive and/or environmentally friendly chromatographic operation due to one or more of the following advantages over conventional chromatographic operations: elimination of a device packing step by the user; elimination of clean-in-place (CIP) steps; elimination of clean-in-place (CIP) steps utilizing sodium hydroxide solution; elimination of any validation steps by the user; and use of a chromatography device comprising biodegradable material. The chromatography media includes porous inorganic particles having a functionalized surface and having a median pore size of at least about 300 Angstroms (A), or at least about 300 A up to about 3000 A. The inorganic particles may have a BET surface area of at least about 20 m2/g, or at least about 25 m2/g, or about 30 m2/g, up to about 2000 m2/g.

公开(公告)号：US11559785B2

公开(公告)日：2023-01-24

申请号：US15521198

申请日：2015-10-22

Applicant: The University of Hull

Inventor： Stephen James Archibald ,  Ping He ,  Stephen John Haswell ,  Nicole Pamme ,  Nathan Joel Brown ,  Mark Duncan Tarn ,  Richard Alexander ,  Mohammad Mehdi Nasr Esfahani

IPC: G01N33/15 ,  G01N30/02 ,  B01J20/28 ,  B01J39/26 ,  B01J41/20 ,  B01J20/283 ,  B01J19/00 ,  G21G1/00 ,  B01J20/291 ,  B01L3/00 ,  B01D15/32 ,  B01D15/36 ,  B01J20/02 ,  B01J20/10 ,  B01J20/22 ,  B01J20/282 ,  B01J20/30 ,  B29C45/14 ,  B29C71/02 ,  G01N30/96 ,  G01N33/00 ,  B29K105/00 ,  B29K709/00

Abstract: The present invention relates to monolithic bodies, uses thereof and processes for the preparation thereof. Certain embodiments of the present invention relate to the use of a monolithic body in the preparation of a radioactive substance, for example a radiopharmaceutical, as part of a microfluidic flow system and a process for the preparation of such a monolithic body.

公开(公告)号：US20220297088A1

公开(公告)日：2022-09-22

申请号：US17832237

申请日：2022-06-03

Applicant: W.R.Grace & Co-CONN.

Inventor： Feng GU

IPC: B01J20/32 ,  B01J39/26 ,  B01J41/20 ,  B01J20/289 ,  B01J41/05 ,  B01J39/17 ,  B01J39/05 ,  B01D15/36 ,  C07K1/18

Abstract: Methods of making functionalized support material am disclosed. Functionalized support material suitable for use in chromatography columns or cartridges, such as in a high pressure liquid chromatography (HPLC) column or a fast protein liquid chromatography (FPLC) column, is also disclosed. Chromatography columns or cartridges containing the functionalized support material, and methods of using functionalized support material, such as a media (e.g., chromatographic material) in a chromatography column or cartridge, are also disclosed.

公开(公告)号：US20220204712A1

公开(公告)日：2022-06-30

申请号：US17601766

申请日：2020-04-07

Applicant: METROHM AG

Inventor： Andreas SEUBERT ,  Jonathan Sebastian TRIPP ,  Rudolf AESCHLIMANN ,  Michael OTT ,  Bastian BRAND

IPC: C08J7/056 ,  B01J47/02 ,  C08J7/12 ,  B01J39/20 ,  B01J39/26 ,  B01J41/14 ,  B01J41/20 ,  B01D15/36

Abstract: A method for modifying a polymer carrier material for use as a stationary phase in an analytical or preparative separating method, the method comprising the steps of: providing a polymer carrier material, which is at least partly formed of aromatic hydrocarbon compounds comprising at least two vinyl or allyl substituents; producing hydroxy groups on/in the polymer carrier material by a method comprising an oxidative treatment of the polymer carrier material and a subsequent reductive or hydrolytic treatment of the reaction product; reacting the product from the previous step with a polyfunctional compound. The invention also relates to a polymer carrier material for use as a stationary phase in an analytical or preparative separating method, in particular a chromatography method, produced according to a method according to the invention.

公开(公告)号：US20210395370A1

公开(公告)日：2021-12-23

申请号：US17465092

申请日：2021-09-02

Applicant: MedImmune Limited

Inventor： Thomas LINKE ,  William K. WANG ,  Ambarish SHAH ,  Hasige SATHISH ,  Alan HUNTER ,  Christopher THOMPSON

IPC: C07K16/28 ,  B01D15/16 ,  B01D15/36 ,  B01J41/14 ,  B01J41/20 ,  A61K47/68 ,  C07K1/18 ,  C07K17/02 ,  A61K39/395 ,  A61K47/02

Abstract: The present invention provides methods for isolating an active polypeptide or immunoconjugate by purification of a solution containing both the active polypeptide or immunoconjugate and an acidic variant thereof, such as a deamidated variant, using anion exchange chromatography. The present invention also provides compositions, formulations, and unit dosage forms comprising the purified polypeptide or immunoconjugate.

公开(公告)号：US11136396B2

公开(公告)日：2021-10-05

申请号：US16786634

申请日：2020-02-10

Applicant: MedImmune Limited

Inventor： Thomas Linke ,  William K. Wang ,  Ambarish Shah ,  Hasige Sathish ,  Alan Hunter ,  Christopher Thompson

IPC: A61K39/00 ,  A61K49/00 ,  A61K39/395 ,  C07K16/28 ,  B01D15/16 ,  B01D15/36 ,  B01J41/14 ,  B01J41/20 ,  A61K47/68 ,  C07K1/18 ,  C07K17/02 ,  A61K47/02

Abstract: The present invention provides methods for isolating an active polypeptide or immunoconjugate by purification of a solution containing both the active polypeptide or immunoconjugate and an acidic variant thereof, such as a deamidated variant, using anion exchange chromatography. The present invention also provides compositions, formulations, and unit dosage forms comprising the purified polypeptide or immunoconjugate.

公开(公告)号：US20210016266A1

公开(公告)日：2021-01-21

申请号：US17022304

申请日：2020-09-16

Applicant: THE BOARD OF REGENTS, UNIVERSITY OF TEXAS SYSTEM ,  DIONEX CORPORATION

Inventor： Purnendu K. DASGUPTA ,  Weixiong HUANG ,  Christopher A. POHL

IPC: B01J39/26 ,  G01N30/60 ,  B01J20/32 ,  B01J20/286 ,  B01D15/36 ,  B01D15/20 ,  B01D15/22 ,  B01J41/20 ,  B01J47/02

Abstract: Open tubular capillary columns for liquid and ion chromatography, based upon an ionically impermeable polyolefin capillary having a bore with a sulfonate-group- or amine-group-functionalized internal surface. The capillary columns may include a coating of ion exchanging nanoparticles electrostatically bound to the functionalized internal surface. The capillary columns may be made by exposing the interior surface to a sulfonating reagent comprising chlorosulfonic acid (CISO3H), preferably from 85 wt % to 95 wt % chlorosulfonic acid at a process temperature of 20 to 25° C. The interior surface may be subsequently exposed to an asymmetrical diamine to form a sulfonic mid-linkage to the diamine, i.e., to form a sulfonamide-linked, amine-group-functionalized internal surface. The coating may be provided by subsequently exposing the interior surface to an aqueous suspension of ion exchanging nanoparticles to electrostatically bond the ion exchanging nanoparticles to the functionalized internal surface.

公开(公告)号：US10800820B2

公开(公告)日：2020-10-13

申请号：US15318286

申请日：2014-06-12

Applicant: Biosyn Arzneimittel GmbH

Inventor： Ortwin Kottwitz ,  Thomas Stiefel ,  Shammana N. Muddukrishna

IPC: C07K14/435 ,  B01D15/36 ,  C07K1/18 ,  B01J41/07 ,  B01J41/20 ,  A61K39/385 ,  C12N7/00 ,  A61K39/00

Abstract: The present invention relates to the provision of a biologically safe hemolymph sera, preferably hemocyanin, more preferably KLH (keyhole limpet hemocyanin). The hemocyanin is purified using anion exchange chromatography.

公开(公告)号：US10751692B2

公开(公告)日：2020-08-25

申请号：US16062445

申请日：2015-12-16

Applicant: Khalifa University of Science and Technology

Inventor： Fawzi Banat ,  Priyabrata Pal ,  Anjali Achazhiyath Edathil

IPC: B01J20/24 ,  B01J20/12 ,  B01J20/20 ,  B01D15/08 ,  B01D15/26 ,  B01J20/34 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28 ,  B01J20/30 ,  B01J41/20 ,  C08B37/00

Abstract: A method of treating a liquid for removal of organic acid anions which comprises contacting a liquid containing organic acid anions with an adsorbent comprising calcium alginate-kaolinite or calcium alginate-quartz and a method of treating a liquid for removal of organic acid anions, heavy metal ions and thermally degraded organic products which comprises contacting a liquid containing organic acid anions, heavy metal ions and thermally degraded organic products with an adsorbent comprising calcium alginate-activated carbon are described.