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    Adeno-associated viral vector-based methods and compositions for introducing an expression cassette into a cell
    2.
    发明授权
    Adeno-associated viral vector-based methods and compositions for introducing an expression cassette into a cell 有权

    公开(公告)号：US07060497B2

    公开(公告)日：2006-06-13

    申请号：US09797858

    申请日：2001-03-01

    申请人： Hiroyuki Nakai ,  Mark A. Kay

    发明人： Hiroyuki Nakai ,  Mark A. Kay

    IPC分类号： C12N15/63 ,  C12N15/00 ,  C12P21/06 ,  A01N63/00 ,  C07H21/02

    CPC分类号： C12N15/86 ,  C12N2750/14143 ,  C12N2750/14151

    摘要： Methods and compositions are provided for introducing an expression cassette into a cell. In the subject methods, a population of at least two distinct adeno-associated viral particles is provided, where each distinct type of viral particle in the population comprises a different portion of the expression cassette to be introduced into the cell. The target cell is contacted with population of adeno-associated viral vectors under conditions sufficient to produce a hetero-concatemer in the cell, where the hetero-concatemer includes a functional expression cassette having an intron that includes an ITR sequence. Also provided by the subject invention are vector preparations for practicing the subject methods as well as kits for use in producing the vectors employed in the subject methods. The subject methods find use in a variety of different gene transfer applications, including both in vivo and in vitro gene transfer applications, and are particularly suited for use in the transfer of long genes.

    Four-stroke-cycle V-type engine of motorcycle
    3.
    发明授权
    Four-stroke-cycle V-type engine of motorcycle 失效
    摩托车四冲程V型发动机

    公开(公告)号：US5743219A

    公开(公告)日：1998-04-28

    申请号：US828591

    申请日：1997-03-31

    申请人： Hiroshi Iio ,  Hiroyuki Nakai

    发明人： Hiroshi Iio ,  Hiroyuki Nakai

    IPC分类号： B60K5/04 ,  F02B61/02 ,  F02B75/02 ,  F02B75/18 ,  F02B75/22 ,  F02F1/24 ,  F02F7/00 ,  F02B77/00

    CPC分类号： F02B61/02 ,  F02B75/22 ,  F02F7/006 ,  F02B2075/027 ,  F02B2075/1808 ,  F02B2275/18 ,  F02F2001/245

    摘要： A four-stroke-cycle engine of a motorcycle arranged between front and rear wheels thereof has a crank case in which a crank shaft is arranged and a plurality of cylinder assemblies operatively connected to the crank shaft and includes a front-side cylinder assembly and a rear-side cylinder assembly along a longitudinal direction of a motorcycle body. Each of the cylinder assemblies is composed of a cylinder block, a cylinder head mounted to a head portion of the cylinder block and a head cover covering the cylinder head. The cylinder assemblies are arranged so as to provide V-shapes along the longitudinal direction of the motorcycle body. A mating surface formed between the head cover and the cylinder head of at least the front-side cylinder assembly of the plural cylinder assemblies has an inclination obliquely downward towards the front wheel with respect to a mating surface formed between the cylinder head and the cylinder block of the front-side cylinder assembly.

    摘要翻译： 布置在其前轮和后轮之间的摩托车的四冲程循环发动机具有曲轴箱，其中布置有曲轴，并且多个气缸组件可操作地连接到曲轴，并且包括前侧气缸组件和 后侧气缸组件沿着摩托车主体的纵向方向。 每个气缸组件由气缸体，安装在气缸体的头部的气缸盖和覆盖气缸盖的气缸盖构成。 气缸组件布置成沿着摩托车主体的纵向方向提供V形。 形成在多个气缸组件的至少前侧气缸组件的头盖和气缸盖之间的配合表面相对于形成在气缸盖和气缸体之间的配合表面向着前轮倾斜向下倾斜 的前侧气缸组件。

    Restorative dental material and method of restoring teeth using same
    5.
    发明授权
    Restorative dental material and method of restoring teeth using same 失效
    修复牙科材料及使用其恢复牙齿的方法

    公开(公告)号：US4764117A

    公开(公告)日：1988-08-16

    申请号：US948360

    申请日：1986-11-24

    申请人： Atsushi Yamashita ,  Toshiaki Yamami ,  Yasuhiro Kondo ,  Hiroyuki Nakai ,  Kazuomi Suzuki

    发明人： Atsushi Yamashita ,  Toshiaki Yamami ,  Yasuhiro Kondo ,  Hiroyuki Nakai ,  Kazuomi Suzuki

    IPC分类号： A61C5/00 ,  A61C5/04 ,  A61C5/08 ,  A61K6/04 ,  A61K6/083 ,  A61C13/00

    CPC分类号： A61K6/046 ,  A61C5/00 ,  A61C5/007 ,  A61K6/0023

    摘要： A restorative dental material is disclosed which comprises a metal casting with an electroplated tin coat containing polymer particles being formed thereon. Such restorative material can be obtained by electroplating the bonding surface of the metal casting using a plating bath containing Sn.sup.++ with polymer particles dispersed therein. An adhesive containing an acid group-containing polymerizable monomer as an adhesive component is applied to the bonding surface having the tin plating layer containing polymer particles and the material is firmly fixed to one or more teeth or other prosthetic appliances.

    摘要翻译： PCT No.PCT / JP86 / 00030 Sec。 371日期：1986年11月24日 102（e）1986年11月24日PCT PCT 1月24日PCT PCT。 出版物WO86 / 05677 日本公报1986年10月9日。公开了一种修复牙科材料，其包括具有在其上形成聚合物颗粒的电镀锡涂层的金属铸件。 使用含有分散在其中的聚合物粒子的含有Sn ++的电镀液，对金属铸件的接合面进行电镀，可以得到这种修复材料。 将含有含酸基的可聚合单体作为粘合剂组分的粘合剂施加到具有含有聚合物颗粒的镀锡层的接合表面上，并将该材料牢固地固定在一个或多个牙齿或其它假体器具上。