公开(公告)号：US07560709B2

公开(公告)日：2009-07-14

申请号：US12011947

申请日：2008-01-30

申请人： Hiroyuki Kimura ,  Kenichi Kusaka ,  Katashi Ishihara

发明人： Hiroyuki Kimura ,  Kenichi Kusaka ,  Katashi Ishihara

IPC分类号： G01N21/64

CPC分类号： G02B21/002 ,  G02B21/16 ,  G02B2207/113

摘要： Provided is a scanning laser microscope including a first laser light source for emitting ultrashort pulsed laser light; a scanning unit for two-dimensionally scanning the ultrashort pulsed laser light on a specimen; a second laser light source for emitting continuous laser light; an irradiation-position adjusting unit for performing two-dimensional adjustment of an irradiation position of the continuous laser light on the specimen; an objective lens for focusing the ultrashort pulsed laser light and the continuous laser light onto the specimen and for collecting fluorescence generated in the specimen; a light-detecting unit for detecting the fluorescence, which is split off from a light path between the objective lens and the scanning unit; and a continuous-laser-light switching unit for permitting radiation of the continuous laser light when the light-detecting unit is not detecting required fluorescence from the specimen on the basis of the ultrashort pulsed laser light.

摘要翻译： 本发明提供一种扫描激光显微镜，其包括用于发射超短脉冲激光的第一激光光源; 用于在样本上二维扫描超短脉冲激光的扫描单元; 用于发射连续激光的第二激光光源; 照射位置调整单元，其对所述检体上的所述连续激光的照射位置进行二维调整; 用于将超短脉冲激光和连续激光聚焦到样本上并用于收集在样本中产生的荧光的物镜; 用于检测从物镜和扫描单元之间的光路分离的荧光的光检测单元; 以及连续激光切换单元，用于当光检测单元基于超短脉冲激光不检测来自样本的所需荧光时，允许连续激光的照射。

公开(公告)号：US20080185533A1

公开(公告)日：2008-08-07

申请号：US12011947

申请日：2008-01-30

申请人： Hiroyuki Kimura ,  Kenichi Kusaka ,  Katashi Ishihara

发明人： Hiroyuki Kimura ,  Kenichi Kusaka ,  Katashi Ishihara

IPC分类号： G01J1/58

CPC分类号： G02B21/002 ,  G02B21/16 ,  G02B2207/113

摘要： Provided is a scanning laser microscope including a first laser light source for emitting ultrashort pulsed laser light; a scanning unit for two-dimensionally scanning the ultrashort pulsed laser light on a specimen; a second laser light source for emitting continuous laser light; an irradiation-position adjusting unit for performing two-dimensional adjustment of an irradiation position of the continuous laser light on the specimen; an objective lens for focusing the ultrashort pulsed laser light and the continuous laser light onto the specimen and for collecting fluorescence generated in the specimen; a light-detecting unit for detecting the fluorescence, which is split off from a light path between the objective lens and the scanning unit; and a continuous-laser-light switching unit for permitting radiation of the continuous laser light when the light-detecting unit is not detecting required fluorescence from the specimen on the basis of the ultrashort pulsed laser light.

摘要翻译： 本发明提供一种扫描激光显微镜，其包括用于发射超短脉冲激光的第一激光光源; 用于在样本上二维扫描超短脉冲激光的扫描单元; 用于发射连续激光的第二激光光源; 照射位置调整单元，其对所述检体上的所述连续激光的照射位置进行二维调整; 用于将超短脉冲激光和连续激光聚焦到样本上并用于收集在样本中产生的荧光的物镜; 用于检测从物镜和扫描单元之间的光路分离的荧光的光检测单元; 以及连续激光切换单元，用于当光检测单元基于超短脉冲激光不检测来自样本的所需荧光时，允许连续激光的照射。

公开(公告)号：US07820958B2

公开(公告)日：2010-10-26

申请号：US12039716

申请日：2008-02-28

申请人： Katashi Ishihara ,  Hitoshi Hatano

发明人： Katashi Ishihara ,  Hitoshi Hatano

IPC分类号： H01S3/10

CPC分类号： G01N21/6445 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/0092

摘要： An improved laser scanning microscope and method of use are disclosed that enable polarization anisotropy measurements to be made with greater sensitivity and accuracy. A controllable optical element is provided in a light path between a laser source and a sample, and is controlled so that the sample is alternately irradiated with light beams that are orthogonally polarized. This enables the signal strength to be higher than with previous laser scanning microscopes. Moreover, because the orthogonally polarized light beams that are alternately incident onto a sample may be switched at a high rate, a reduction may be achieved in the influence of molecular motion within a sample in which high speed biological reactions are observed.

摘要翻译： 公开了一种改进的激光扫描显微镜和使用方法，其能够以更高的灵敏度和精度进行偏振各向异性测量。 可控光学元件设置在激光源和样品之间的光路中，并被控制，使得样品被正交极化的光束交替地照射。 这使得信号强度比先前的激光扫描显微镜高。 此外，由于交替入射到样品上的正交偏振光束可以以高速率被切换，所以在观察到高速生物反应的样品内的分子运动的影响中可以实现减小。

公开(公告)号：US20080225906A1

公开(公告)日：2008-09-18

申请号：US12039716

申请日：2008-02-28

申请人： Katashi Ishihara ,  Hitoshi Hatano

发明人： Katashi Ishihara ,  Hitoshi Hatano

IPC分类号： H01S3/10

CPC分类号： G01N21/6445 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/0092

摘要： An improved laser scanning microscope and method of use are disclosed that enable polarization anisotropy measurements to be made with greater sensitivity and accuracy. A controllable optical element is provided in a light path between a laser source and a sample, and is controlled so that the sample is alternately irradiated with light beams that are orthogonally polarized. This enables the signal strength to be higher than with previous laser scanning microscopes. Moreover, because the orthogonally polarized light beams that are alternately incident onto a sample may be switched at a high rate, a reduction may be achieved in the influence of molecular motion within a sample in which high speed biological reactions are observed.

摘要翻译： 公开了一种改进的激光扫描显微镜和使用方法，其能够以更高的灵敏度和精度进行偏振各向异性测量。 可控光学元件设置在激光源和样品之间的光路中，并被控制，使得样品被正交极化的光束交替地照射。 这使得信号强度比先前的激光扫描显微镜高。 此外，由于交替入射到样品上的正交偏振光束可以以高速率被切换，所以在观察到高速生物反应的样品内的分子运动的影响中可以实现减小。