公开(公告)号：US11926845B2

公开(公告)日：2024-03-12

申请号：US17162207

申请日：2021-01-29

Applicant: Hitachi, Ltd.

Inventor： Yuki Niimi ,  Hiroko Hanzawa ,  Maiko Tanabe ,  Kunio Ohyama ,  Shizu Takeda

IPC: C12N5/073 ,  C12M1/36

CPC classification number: C12N5/0605 ,  C12M41/48 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/00 ,  C12N2533/30 ,  C12N2535/00

Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.