公开(公告)号：US11926845B2

公开(公告)日：2024-03-12

申请号：US17162207

申请日：2021-01-29

Applicant: Hitachi, Ltd.

Inventor： Yuki Niimi ,  Hiroko Hanzawa ,  Maiko Tanabe ,  Kunio Ohyama ,  Shizu Takeda

IPC: C12N5/073 ,  C12M1/36

CPC classification number: C12N5/0605 ,  C12M41/48 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/00 ,  C12N2533/30 ,  C12N2535/00

Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.

公开(公告)号：US09421284B2

公开(公告)日：2016-08-23

申请号：US14235005

申请日：2012-12-26

Applicant: Hitachi, Ltd.

Inventor： Nami Sugita ,  Naomi Manri ,  Wataru Takeuchi ,  Yuichi Morimoto ,  Hiroko Hanzawa

IPC: B01J19/24 ,  A61K51/08 ,  B01J19/00 ,  B01L3/00 ,  B01F13/00 ,  C07B59/00

CPC classification number: A61K51/088 ,  B01F13/0062 ,  B01F13/0066 ,  B01J19/0006 ,  B01J19/0093 ,  B01J19/24 ,  B01J2219/00049 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00842 ,  B01J2219/00873 ,  B01J2219/0088 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00905 ,  B01J2219/00957 ,  B01J2219/00961 ,  B01J2219/00963 ,  B01J2219/00986 ,  B01J2219/24 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0887 ,  C07B59/00 ,  Y10T436/12

Abstract: A reaction container in which to mix a first chemical compound with a second chemical compound has a main body and a lid member formed oppositely on a top face side of the main body; a flow channel on the top face of the main body; and a labeling agent solidification section at an intermediate section of the flow channel to remove a solvent in a solution of the second chemical compound and solidify the second chemical compound. First and second chemical compound supply sections and a mixture discharge section are formed on the upstream and downstream sides of the labeling agent solidification section, respectively. The reactor is provided with a liquid sending unit to supply the first and second chemical compounds and reciprocally send a solution of the first chemical compound to an upper part of the second chemical compound solidified at the solidification section.

Abstract translation: 将第一化合物与第二化合物混合的反应容器具有主体和在主体的顶面相对地形成的盖部件; 主体顶面上的流路; 以及在流路的中间部分处的标记剂固化部分，以除去第二化合物的溶液中的溶剂并固化第二化合物。 分别在标签试剂凝固部的上游侧和下游侧形成第一和第二化合物供给部和混合物排出部。 反应器设有一个送液单元，用于供应第一和第二化合物，并将第一化合物的溶液往凝固段固化的第二化合物的上部相互转移。

公开(公告)号：US11566232B2

公开(公告)日：2023-01-31

申请号：US16122912

申请日：2018-09-06

Applicant: HITACHI, LTD. ,  The University of Tokyo

Inventor： Maiko Tanabe ,  Shizu Takeda ,  Masahiro Okanojo ,  Hiroko Hanzawa ,  Masao Washizu ,  Kennedy Omondi Okeyo

IPC: C12N5/074 ,  C12N5/00 ,  C12M1/12 ,  C12M1/00

Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.

公开(公告)号：US10947490B2

公开(公告)日：2021-03-16

申请号：US15896947

申请日：2018-02-14

Applicant: HITACHI, LTD.

Inventor： Hikaru Saito ,  Masaharu Kiyama ,  Midori Kato ,  Kunio Ohyama ,  Guangbin Zhou ,  Hiroko Hanzawa ,  Shizu Takeda

IPC: C12M1/00 ,  C12M1/36 ,  C12M1/34 ,  C12M1/12 ,  C12M3/00

Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.

公开(公告)号：US09952213B2

公开(公告)日：2018-04-24

申请号：US14514523

申请日：2014-10-15

Applicant: HITACHI, LTD.

Inventor： Hiroko Hanzawa ,  Takeshi Sakamoto ,  Yuji Kuge

IPC: G01N33/50 ,  G01N33/53 ,  A61P9/10 ,  G01N33/564 ,  C07K14/47 ,  C07K14/745 ,  C07K16/36

CPC classification number: G01N33/564 ,  A61P9/10 ,  C07K14/472 ,  C07K14/745 ,  C07K16/36 ,  G01N33/50 ,  G01N33/53 ,  G01N2333/4716 ,  G01N2800/324

Abstract: It is intended to evaluate an ischemic heart disease with high accuracy by convenient operation. The method for evaluating an ischemic heart disease according to the present invention comprises the steps of: assaying complement factor H and/or complement factor D in a sample derived from the blood of a test subject; and comparing the concentration of the complement factor H and/or the concentration of the complement factor D assayed in the preceding step with a reference value(s), wherein it is determined that the seriousness of the ischemic heart disease is high when the concentration falls below the reference value.

公开(公告)号：US09575074B2

公开(公告)日：2017-02-21

申请号：US14935994

申请日：2015-11-09

Applicant: Hitachi, Ltd. ,  National University Corporation Hokkaido University

Inventor： Takeshi Sakamoto ,  Hiroko Hanzawa ,  Naomi Manri ,  Yuji Kuge

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/68 ,  A61B5/02 ,  A61B5/055 ,  A61B5/00 ,  A61B6/00 ,  A61B8/08

CPC classification number: G01N33/6893 ,  A61B5/0071 ,  A61B5/02007 ,  A61B5/055 ,  A61B6/504 ,  A61B6/508 ,  A61B8/0891 ,  G01N2800/323 ,  G01N2800/52

Abstract: The present invention provides a method and a means for evaluating atherosclerotic lesions by identifying a protein (marker protein) group, the expression level of which varies according to the progression of the atherosclerotic lesion, and using the proteins. Specifically, the present invention relates to a method for evaluating atherosclerotic lesions, comprising the steps of detecting a marker protein exhibiting an expression pattern (expression variation) characteristic at a specific disease stage of atherosclerotic lesions in a subject, and evaluating the atherosclerotic lesions in the subject based on the detection result.

Abstract translation: 本发明提供了一种通过鉴定其表达水平根据动脉粥样硬化病变的进展以及使用蛋白质而变化的蛋白质（标记蛋白）组来评价动脉粥样硬化病变的方法和手段。 具体而言，本发明涉及评价动脉粥样硬化病变的方法，其特征在于，包括以下步骤：检测在受试者的动脉粥样硬化病变的特定疾病阶段具有特征的表达模式（表达变异）的标记蛋白，并评价动脉粥样硬化病变 主体根据检测结果。