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公开(公告)号：US12165744B2
公开(公告)日：2024-12-10
申请号：US16824365
申请日：2020-03-19
Inventor： Maiko Tanabe , Shizu Takeda , Kiyoto Ito , Osamu Imaichi , Kenji Tsuge , Michihiro Araki
Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.
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Patent Agency Ranking
公开(公告)号：US11566232B2
公开(公告)日：2023-01-31
申请号：US16122912
申请日：2018-09-06
Applicant: HITACHI, LTD. , The University of Tokyo
Inventor： Maiko Tanabe , Shizu Takeda , Masahiro Okanojo , Hiroko Hanzawa , Masao Washizu , Kennedy Omondi Okeyo
Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.
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公开(公告)号：US10947490B2
公开(公告)日：2021-03-16
申请号：US15896947
申请日：2018-02-14
Applicant: HITACHI, LTD.
Inventor： Hikaru Saito , Masaharu Kiyama , Midori Kato , Kunio Ohyama , Guangbin Zhou , Hiroko Hanzawa , Shizu Takeda
Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.
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公开(公告)号：US11926845B2
公开(公告)日：2024-03-12
申请号：US17162207
申请日：2021-01-29
Applicant: Hitachi, Ltd.
Inventor： Yuki Niimi , Hiroko Hanzawa , Maiko Tanabe , Kunio Ohyama , Shizu Takeda
CPC classification number: C12N5/0605 , C12M41/48 , C12N2501/999 , C12N2506/45 , C12N2533/00 , C12N2533/30 , C12N2535/00
Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.
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公开(公告)号：US10329525B2
公开(公告)日：2019-06-25
申请号：US15323977
申请日：2014-07-23
Applicant: Hitachi, Ltd.
Inventor： Masaharu Kiyama , Guangbin Zhou , Takayuki Nozaki , Ryota Nakajima , Daisuke Suzuki , Yumiko Igarashi , Shizu Takeda , Taku Nakamura , Masakazu Sugaya , Koichi Terada
Abstract: There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe 7 is connected with a gas bag 14, the liquid to be fed into the feed pipe 7 is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container 8 for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.
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公开(公告)号：US10087410B2
公开(公告)日：2018-10-02
申请号：US14770627
申请日：2013-03-25
Applicant: Hitachi, Ltd.
Inventor： Takayuki Nozaki , Guangbin Zhou , Masaharu Kiyama , Taku Nakamura , Shizu Takeda , Ryota Nakajima , Masakazu Sugaya , Koichi Terada
Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.
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