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公开(公告)号：US09862993B2
公开(公告)日：2018-01-09
申请号：US15028907
申请日：2013-10-21
Applicant: HITACHI, LTD.
Inventor： Maiko Tanabe , Hideki Kambara , Masataka Shirai
CPC classification number: C12Q1/6837 , B01L3/5085 , B01L2300/0819 , B01L2300/0893 , C12Q1/6809 , C12Q2521/501 , C12Q2531/113 , C12Q2545/114 , C12Q2565/601 , C12Q2565/629 , C12Q2565/631
Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a genetic analysis system, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.
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公开(公告)号：US11926845B2
公开(公告)日：2024-03-12
申请号：US17162207
申请日：2021-01-29
Applicant: Hitachi, Ltd.
Inventor： Yuki Niimi , Hiroko Hanzawa , Maiko Tanabe , Kunio Ohyama , Shizu Takeda
CPC classification number: C12N5/0605 , C12M41/48 , C12N2501/999 , C12N2506/45 , C12N2533/00 , C12N2533/30 , C12N2535/00
Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.
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Patent Agency Ranking
公开(公告)号：US11680286B2
公开(公告)日：2023-06-20
申请号：US17323311
申请日：2021-05-18
Applicant: Hitachi, Ltd.
Inventor： Masataka Shirai , Hideki Kambara , Kiyomi Taniguchi , Maiko Tanabe
IPC: C12Q1/6837 , C12N15/10
CPC classification number: C12Q1/6837 , C12N15/1096 , C12Q1/6837 , C12Q2525/155 , C12Q2565/537 , C12Q2565/601
Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.
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4.发明授权公开(公告)号：US10030240B2
公开(公告)日：2018-07-24
申请号：US14771339
申请日：2013-03-12
Applicant: Hitachi, Ltd.
Inventor： Masataka Shirai , Hideki Kambara , Kiyomi Taniguchi , Maiko Tanabe
IPC: C12N15/10 , C12Q1/6874 , B01L3/00 , B01L7/00
Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.
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公开(公告)号：US20160251705A1
公开(公告)日：2016-09-01
申请号：US15028907
申请日：2013-10-21
Applicant: HITACHI, LTD.
Inventor： Maiko Tanabe , Hideki Kambara , Masataka Shirai
CPC classification number: C12Q1/6837 , B01L3/5085 , B01L2300/0819 , B01L2300/0893 , C12Q1/6809 , C12Q2521/501 , C12Q2531/113 , C12Q2545/114 , C12Q2565/601 , C12Q2565/629 , C12Q2565/631
Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a means, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.
Abstract translation: 为了对大量细胞中的大量基因的任意序列区进行解码，有必要在各个片段中对核酸进行片段化并引入每个细胞不同的序列。 然而，在用于分析大量细胞的常规构造中，存在在可以引入每个区域独特的标签序列之前，将不同区域的切割片段混合的问题。 本发明构建成还包括当捕获从底物上多个区域中的细胞提取的核酸并合成和分段针对每个单独区域的核酸的互补DNA链（cDNA）时的手段，以立即引入标签 每个区域对于所述片段唯一的序列。
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公开(公告)号：US12165744B2
公开(公告)日：2024-12-10
申请号：US16824365
申请日：2020-03-19
Inventor： Maiko Tanabe , Shizu Takeda , Kiyoto Ito , Osamu Imaichi , Kenji Tsuge , Michihiro Araki
Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.
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公开(公告)号：US11566232B2
公开(公告)日：2023-01-31
申请号：US16122912
申请日：2018-09-06
Applicant: HITACHI, LTD. , The University of Tokyo
Inventor： Maiko Tanabe , Shizu Takeda , Masahiro Okanojo , Hiroko Hanzawa , Masao Washizu , Kennedy Omondi Okeyo
Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.
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