公开(公告)号：US06346394B1

公开(公告)日：2002-02-12

申请号：US09055210

申请日：1998-04-06

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N924

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85° C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 该酶具有令人满意的高的热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有助于以工业规模生产海藻糖， 以令人满意的高产量。

公开(公告)号：US5856146A

公开(公告)日：1999-01-05

申请号：US505377

申请日：1995-07-21

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/24 ,  C12N15/52 ,  C12N15/56 ,  C12N15/70 ,  C12P1/04 ,  C12P19/12 ,  C12P19/14 ,  C12P21/00 ,  C12R1/01 ,  C12R1/19 ,  C07K14/195 ,  C12P19/18

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 该酶具有令人满意的高的热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。

公开(公告)号：US6027918A

公开(公告)日：2000-02-22

申请号：US798269

申请日：1997-02-11

申请人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

发明人： Hitoshi Mitsuzumi ,  Michio Kubota ,  Toshiyuki Sugimoto

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/24 ,  C12N15/52 ,  C12N15/56 ,  C12N15/70 ,  C12P1/04 ,  C12P19/12 ,  C12P19/14 ,  C12P21/00 ,  C12R1/01 ,  C12R1/19 ,  C12P21/02 ,  C12N15/31

CPC分类号： C12Y302/01141 ,  C12N9/1051 ,  C12P19/12 ,  C12P19/14 ,  C12Y204/01064 ,  Y10S435/822 ,  Y10S530/825

摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.

摘要翻译： 公开了一种重组热稳定酶，其分子量为约54,000-64,000道尔顿，pI为约5.6-6.6，并且从具有海藻糖结构的末端单元和葡萄糖聚合度的非还原性糖释放海藻糖 至少3.酶具有令人满意的高热稳定性，即使在85℃下在水溶液（pH7.0）中孵育60分钟也不会基本上灭活，这有利于在工业规模上生产海藻糖， 以令人满意的高产量。

公开(公告)号：US20080221045A1

公开(公告)日：2008-09-11

申请号：US11722735

申请日：2005-11-21

申请人： Hitoshi Mitsuzumi ,  Mika Takami ,  Yoshikatsu Miwa ,  Hiroto Chaen ,  Toshio Miyake

发明人： Hitoshi Mitsuzumi ,  Mika Takami ,  Yoshikatsu Miwa ,  Hiroto Chaen ,  Toshio Miyake

IPC分类号： A61K31/7048 ,  C07H17/04 ,  A23L2/00 ,  A23F3/00

CPC分类号： A61K31/7048 ,  A23F3/163 ,  A23L2/52 ,  A23L33/10

摘要： An object of the present invention is to provide a means for improving hepatic function of mammals including humans to normal condition. The present invention achieves the above object by providing a hepatic function-improving agent comprising α-glucosyl-hesperidin as an effective ingredient.

摘要翻译： 本发明的目的是提供一种将包括人的哺乳动物的肝功能改善至正常状况的手段。 本发明通过提供包含α-葡萄糖基 - 橙皮苷作为有效成分的肝功能改善剂来实现上述目的。