公开(公告)号：US20090186343A1

公开(公告)日：2009-07-23

申请号：US12035352

申请日：2008-02-21

申请人： Hongyi Wang ,  Norha Deluge ,  Kristi Kincaid ,  Yuri Belosludtsev ,  Tommie Lincecum, JR. ,  Amy Williams ,  Amy Bryant ,  Ming Fa ,  Benjamin Stevens ,  Susan H. Hardin

发明人： Hongyi Wang ,  Norha Deluge ,  Kristi Kincaid ,  Yuri Belosludtsev ,  Tommie Lincecum, JR. ,  Amy Williams ,  Amy Bryant ,  Ming Fa ,  Benjamin Stevens ,  Susan H. Hardin

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C07H1/00 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q1/6869 ,  C12Q2565/101 ,  C12Q2525/117 ,  C12Q2521/101

摘要： A novel and efficient single pot synthetic schemes are disclosed for preparing modified nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs. The novel method is used to prepare nucleotides, nucleotide analogs, nucleotide polyphosphates, and nucleotide polyphosphate analogs having non-persistent or persistent and non-persistent modifications. Novel biomolecular reactions are also disclosed using the novel modified biomolecules disclosed herein.

摘要翻译： 公开了一种用于制备修饰的核苷酸，核苷酸类似物，核苷酸多磷酸酯和核苷酸多磷酸酯类似物的新型和有效的单锅合成方案。 该新方法用于制备具有非持久性或持久性和非持久性修饰的核苷酸，核苷酸类似物，核苷酸多磷酸酯和核苷酸多磷酸酯类似物。 使用本文公开的新的改性生物分子也公开了新的生物分子反应。

公开(公告)号：US20110165652A1

公开(公告)日：2011-07-07

申请号：US12812952

申请日：2009-01-14

申请人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

发明人： Susan H. Hardin ,  Tommie Lloyd Lincecum, JR. ,  Norha Deluge ,  Hongyi Wang ,  Yuri Belosludtsev ,  Kristi Kincaid ,  Anelia Kraltcheva ,  Benjamin Stevens ,  Ming Fa ,  Amy Bryant ,  Amy Castillo ,  Hye Eun Kim ,  Uma Nagaswamy ,  Mitsu Sreedhar Reddy ,  Alok N. Bandekar ,  Ivan Pan ,  Andrei Volkov

IPC分类号： C12N9/12

CPC分类号： C07H19/207 ,  C07H19/10 ,  C12N9/1252 ,  C12Q1/6869

摘要： Compositions, systems and methods of sequencing are disclosed, where the compositions and systems include polymerase enzymes that have been genetically modified to more efficiently incorporate nucleotides including labels having a detectable properties that are released during incorporation, to augment a rate of labeled nucleotide incorporation, to augment a rate of pyrophosphate release, or to augment two or more of these properties and rates. Also disclosed are terminally labeled and dual labeled nucleotides, and click-chemistry based methods of synthesizing the same.

摘要翻译： 公开了组合物，系统和测序方法，其中组合物和系统包括经遗传修饰以更有效地掺入核苷酸的聚合酶，包括在掺入期间释放的具有可检测性质的标记，以增加标记的核苷酸掺入率， 增加焦磷酸盐释放的速率，或增加这些性质和速率中的两种或更多种。 还公开了末端标记和双重标记的核苷酸，以及基于点击化学的合成方法。