公开(公告)号：US06596505B2

公开(公告)日：2003-07-22

申请号：US09835562

申请日：2001-04-17

Applicant: Howard Ceri ,  Merle Edwin Olson

Inventor： Howard Ceri ,  Merle Edwin Olson

IPC: C12Q102

CPC classification number: C12M25/00 ,  C12M23/12 ,  C12M25/06

Abstract: The present invention relates to an apparatus and methods for testing the formation of biofilms on various materials. The apparatus includes a lid and a vessel, wherein the lid may be configured to accept various materials for the testing of biofilm formation. For example, the lid may contain a plurality of projections onto which materials may be coated or disposed. The vessel is adapted to receive the lid in a fluid tight communication and to retain a liquid growth medium therein. After a material has been disposed upon the projections, the material is suspended within the vessel containing the liquid growth medium. The material is allowed to incubate for a period of time in which a biofilm forms upon the material. The material is then removed from the liquid growth medium and the biofilms formed thereupon are used to test the efficiency of various biocides.

Abstract translation: 本发明涉及用于测试各种材料上生物膜形成的装置和方法。 该装置包括盖子和容器，其中盖子可被配置为接受用于生物膜形成测试的各种材料。 例如，盖可以包含多个突起物，材料可以涂覆在其上。 容器适于以不透气的方式接纳盖并将液体生长介质保持在其中。 在将材料放置在突起上之后，将材料悬浮在容纳液体生长介质的容器内。 允许材料在材料上形成生物膜的一段时间内孵育。 然后将材料从液体生长培养基中除去，并且其上形成的生物膜用于测试各种杀生物剂的效率。

公开(公告)号：US06051423A

公开(公告)日：2000-04-18

申请号：US126957

申请日：1998-07-31

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: B01L3/00 ,  C12M1/14 ,  C12M1/26 ,  C12M1/32 ,  C12M1/34 ,  C12N9/20 ,  C12M1/20

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Bacteria are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Bacteria are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antibacterial reagent may be determined by treating the projections with antibacterial reagent before carrying out the assay, by treating each row of projections with a different antibacterial reagent, and each of the projections in a row with a different concentration of antibacterial reagent. A biofilm assay device includes a vessel including at least one channel for flow of liquid growth medium, projections arranged in at least one row and having a support for supporting the projections within the channel, and a tilt table to flow liquid growth medium along each channel in different directions across the projections. A further biofilm assay device includes a vessel including channels for flow of liquid growth medium, projections arranged in rows and having a support for supporting the projections within the channels, and a rocking table to rock the vessel and flow liquid growth medium along each channel across the projections.

Abstract translation: 将细菌孵育以在突起上形成生物膜，通过提供横跨突起的液体生长培养基流动，液体流动方向被重复改变，以及由所得生物膜制成的测定。 培养细菌以在排列成排的突起上形成生物膜，每行中有几个突起，同时在每排突起处提供液体生长培养基流，以及由所得生物膜制成的测定。 生物膜对抗菌试剂的敏感性可以通过在进行测定之前用抗菌试剂处理突起来确定，通过用不同的抗菌试剂处理每一排突起，并且每个突起以不同浓度的抗菌试剂 。 生物膜测定装置包括容器，其包括用于液体生长介质流动的至少一个通道，布置在至少一排中的突起，并具有用于支撑通道内的突起的支撑件，以及倾斜台，用于沿着每个通道流动液体生长介质 在不同的方向跨越投影。 另外的生物膜测定装置包括容器，该容器包括用于液体生长介质流动的通道，排列成行的突起物，并具有用于支撑通道内的突起的支撑件，以及摇摆台，用于沿每个通道跨过容器和流动液体生长介质 预测。

公开(公告)号：US06599714B1

公开(公告)日：2003-07-29

申请号：US08614593

申请日：1996-03-13

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: C12Q118

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Bacteria are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Bacteria are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antibacterial reagent may be determined by treating the projections with antibacterial reagent before carrying out the assay, by treating each row of projections with a different antibacterial reagent, and each of the projections in a row with a different concentration of antibacterial reagent.

Abstract translation: 将细菌孵育以在突起上形成生物膜，通过提供横跨突起的液体生长培养基流动，液体流动方向被重复改变，以及由所得生物膜制成的测定。 培养细菌以在排列成排的突起上形成生物膜，每行中有几个突起，同时在每排突起处提供液体生长培养基流，以及由所得生物膜制成的测定。 生物膜对抗菌试剂的敏感性可以通过在进行测定之前用抗菌试剂处理突起来确定，通过用不同的抗菌试剂处理每一排突起，并且每个突起以不同浓度的抗菌试剂 。

公开(公告)号：US06326190B1

公开(公告)日：2001-12-04

申请号：US09484527

申请日：2000-01-18

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: C12M120

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Bacteria are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Bacteria are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antibacterial reagent may be determined by treating the projections with antibacterial reagent before carrying out the assay, by treating each row of projections with a different antibacterial reagent, and each of the projections in a row with a different concentration of antibacterial reagent. A biofilm assay device includes a vessel including at least one channel for flow of liquid growth medium, projections arranged in at least one row and having a support for supporting the projections within the channel, and a tilt table to flow liquid growth medium along each channel in different directions across the projections. A further biofilm assay device includes a vessel including channels for flow of liquid growth medium, projections arranged in rows and having a support for supporting the projections within the channels, and a rocking table to rock the vessel and flow liquid growth medium along each channel across the projections.

Abstract translation: 将细菌孵育以在突起上形成生物膜，通过提供横跨投影的液体生长培养基流动，重复改变液体流动的方向，以及由生成的生物膜制成的测定。 培养细菌以在排列成排的突起上形成生物膜，每行中有几个突起，同时在每排突起处提供液体生长培养基流，以及由所得生物膜制成的测定。 生物膜对抗菌试剂的敏感性可以通过在进行测定之前用抗菌试剂处理突起来确定，通过用不同的抗菌试剂处理每一排突起，并且每个突起以不同浓度的抗菌试剂 。 生物膜测定装置包括容器，其包括用于液体生长介质流动的至少一个通道，布置在至少一排中的突起，并具有用于支撑通道内的突起的支撑件，以及倾斜台，用于沿着每个通道流动液体生长介质 在不同的方向跨越投影。 另外的生物膜测定装置包括容器，其包括用于液体生长介质流动的通道，排列成行的突起物，并具有用于支撑通道内的突起的支撑件，以及摇摆台，用于沿每个通道跨过容器和流动液体生长介质 预测。

公开(公告)号：US07041470B2

公开(公告)日：2006-05-09

申请号：US10106115

申请日：2002-03-27

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: C12Q1/18

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Biofilm forming organisms are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Biofilm forming organisms are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antimicrobial reagent may be determined by treating the projections with antimicrobial reagent before carrying out the assay, by treating each row of projections with a different antimicrobial reagent, and each of the projections in a row with a different concentration of antimicrobial reagent.

Abstract translation: 将生物膜形成生物体孵育以在突起上形成生物膜，通过提供液体生长培养基跨过突起的流动，液体的流动方向被重复改变，以及由所得生物膜制成的测定。 将生物膜形成生物体孵育以在排列成排的突起上形成生物膜，每行中具有若干突起，同时在每排突起处提供液体生长介质流，以及由所得生物膜制成的测定。 生物膜对抗微生物试剂的敏感性可以通过在进行测定之前用抗微生物剂处理突出物，通过用不同的抗微生物试剂处理每一排突起，并且将每个突起以不同浓度的抗微生物剂 。

公开(公告)号：US06599696B2

公开(公告)日：2003-07-29

申请号：US10139586

申请日：2002-05-02

Applicant: Merle Edwin Olson ,  Howard Ceri

Inventor： Merle Edwin Olson ,  Howard Ceri

IPC: C12Q100

CPC classification number: C12M25/00 ,  C12M23/12 ,  C12M25/06

Abstract: A method for testing the performance of surface coatings on the formation of biofilm and encrustation on materials. The method includes, providing a plurality of material retaining sites, providing the material retaining sites with a material, wherein the material models a surface likely to be involved in biofilm formation and encrustation deposit. The method further includes the steps of providing a liquid growth medium, wherein the liquid growth medium includes at least one microorganism, the liquid growth medium arranged to cover at least a portion of the material, and incubating microorganism and encrustation on the material retaining sites in the presence of the liquid growth medium.

Abstract translation: 一种用于测试表面涂层对材料形成生物膜和结垢的性能的方法。 该方法包括提供多个材料保留位置，为材料保留位置提供材料，其中该材料模拟可能参与生物膜形成和结垢沉积的表面。 该方法还包括提供液体生长培养基的步骤，其中液体生长培养基包括至少一种微生物，液体生长培养基布置成覆盖材料的至少一部分，并将微生物和结垢保温在材料保留位置上 存在液体生长培养基。

公开(公告)号：US06410256B1

公开(公告)日：2002-06-25

申请号：US09380714

申请日：2000-07-19

Applicant: Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

Inventor： Howard Ceri ,  Merle Edwin Olson ,  Douglas Walter Morck ,  Ronald Rae Read ,  Andre Gerald Buret

IPC: C12Q102

CPC classification number: B01L3/50853 ,  C12M23/38 ,  C12M25/06 ,  C12M27/16 ,  C12M35/04 ,  C12N9/20

Abstract: Biofilm forming organisms are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Biofilm forming organisms are incubated to form a biofilm on projections arranged in rows, with several projections in each row, while providing a flow of liquid growth medium across each row of projections, and an assay made of the resulting biofilm. Sensitivity of the biofilm to antimicrobial reagent may be determined by treating the projections with antimicrobial reagent before carrying out the assay, by treating each row of projections with a different antimicrobial reagent, and each of the projections in a row with a different concentration of antimicrobial reagent.

Abstract translation: 将生物膜形成生物体孵育以在突起上形成生物膜，通过提供液体生长培养基跨过突起的流动，液体的流动方向被重复改变，以及由所得生物膜制成的测定。 将生物膜形成生物体孵育以在排列成排的突起上形成生物膜，每行中具有若干突起，同时在每排突起处提供液体生长介质流，以及由所得生物膜制成的测定。 生物膜对抗微生物试剂的敏感性可以通过在进行测定之前用抗微生物剂处理突出物，通过用不同的抗微生物试剂处理每一排突起，并且将每个突起以不同浓度的抗微生物剂 。