公开(公告)号：US5106740A

公开(公告)日：1992-04-21

申请号：US622115

申请日：1990-12-06

申请人： Hubert Bader ,  Hans-Ullrich Hoppe ,  Michael Magerstadt ,  Merten Schlingmann ,  Dieter Ulschneider ,  Axel Walch

发明人： Hubert Bader ,  Hans-Ullrich Hoppe ,  Michael Magerstadt ,  Merten Schlingmann ,  Dieter Ulschneider ,  Axel Walch

IPC分类号： C07H19/207 ,  C12M1/40 ,  C12N11/04 ,  C12N11/08

CPC分类号： C12M21/18 ,  C07H19/207 ,  C12M25/02 ,  C12N11/04 ,  C12N11/08

摘要： A cofactor having an aromatic or benzylic amino group is converted into an isothiocyanate by reaction with a compound such as thiophosgene and the isothiocyanate of the cofactor is attached to a polymer which is preferably water-soluble. When the polymer has amino groups, a thiourea bridge is formed between the polymer and cofactor and a polymer having hydroxyl groups results in a thiocarbamate bridge. The polymer may be a copolymer of vinylamine or vinylmethylamine and vinylmethylacetamide, partially alkylamine-substituted .alpha., .beta.-poly-(2-hydroxyethyl)-D,L-aspartamide, polyethyleneimine or carbohydrate. The cofactor bound to a polymer and an enzyme are contained in microcapsules or in a membrane to form an enzyme reactor.

摘要翻译： 具有芳族或苄基氨基的辅因子通过与化合物例如硫代光气反应而转化成异硫氰酸酯，辅因子的异硫氰酸酯连接到优选水溶性的聚合物上。 当聚合物具有氨基时，在聚合物和辅因子之间形成硫脲桥，并且具有羟基的聚合物导致硫代氨基甲酸酯桥。 聚合物可以是乙烯胺或乙烯基甲胺和乙烯基甲基乙酰胺，部分烷基胺取代的+60，β-聚 - （2-羟乙基）-D，L-天冬酰胺，聚乙烯亚胺或碳水化合物的共聚物。 与聚合物和酶结合的辅因子包含在微胶囊或膜中以形成酶反应器。