公开(公告)号：US5317090A

公开(公告)日：1994-05-31

申请号：US989902

申请日：1992-12-11

申请人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

发明人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

IPC分类号： C07K14/705 ,  C12Q1/68 ,  A61K35/14

CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

公开(公告)号：US5468617A

公开(公告)日：1995-11-21

申请号：US190555

申请日：1994-02-02

申请人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

发明人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

IPC分类号： C07K14/705 ,  C12Q1/68 ,  G01N33/48

CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

摘要翻译： 将以前分离的乙肝病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp的细胞DNA片段中作为探针，从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。 RAR-β基因由视黄酸（RA）转录上调，其启动子区域可能含有RARE（视黄酸反应元件）。