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    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor
    3.
    发明授权
    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor 失效
    类固醇/甲状腺激素受体相关基因，其在人肝细胞癌中不适当地表达，并且其是视黄酸受体

    公开(公告)号：US5376530A

    公开(公告)日：1994-12-27

    申请号：US95706

    申请日：1993-07-22

    申请人： Hughes B. De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    发明人： Hughes B. De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    IPC分类号： C07K14/705 ,  C12Q1/68

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

    摘要翻译： 将以前分离的乙型肝炎病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp细胞DNA片段中作为探针从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。 RAR-β基因由视黄酸（RA）转录上调，其启动子区域可能含有RARE（视黄酸反应元件）。

    Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it
    6.
    发明授权
    Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it 失效
    用于生产包含乙型肝炎病毒基因组的DNA和包含它的载体的方法

    公开(公告)号：US06225458B1

    公开(公告)日：2001-05-01

    申请号：US08347162

    申请日：1994-11-23

    申请人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Patrick Charnay

    发明人： Pierre Tiollais ,  Alex Fritsch ,  Christine Pourcel ,  Patrick Charnay

    IPC分类号： A61K3929

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122 ,  C12Q1/706

    摘要： The invention relates to a process for producing DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. The invention also relates to vectors containing said cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B.

    摘要翻译： 本发明涉及一种对应于乙型肝炎病毒DNA的DNA的方法。 它包括在细菌中克隆后面的DNA，其在先前在聚合酶存在下通过相应的前体核苷酸修复。 本发明还涉及在其基因组中含有所述克隆的DNA的载体。 克隆的DNA可用作检测生物样品中特别是血液或血浆中B型肝炎病毒存在的探针。 其在细菌中的表达提供了含有针对乙型肝炎疫苗接种性质的蛋白质片段的杂合蛋白质。

    Production of interferon
    7.
    发明授权
    Production of interferon 失效
    生产干扰素

    公开(公告)号：US5541312A

    公开(公告)日：1996-07-30

    申请号：US425934

    申请日：1982-09-28

    申请人： Michel Revel ,  Pierre Tiollais

    发明人： Michel Revel ,  Pierre Tiollais

    IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C07K14/54 ,  C07K14/565 ,  C12N15/00 ,  C12N15/22 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

    CPC分类号： C07K14/5412 ,  C07K14/565

    摘要： The present invention .relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in .beta.1 highly purified form, to the mRNA of human interferon in .sym.2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon .beta.1 in highly purified form, and human interferon .beta.2 in highly purified form.

    摘要翻译： 本发明涉及分离含有人成纤维细胞中编码干扰素的核苷酸序列的遗传物质（DNA）的方法，其包括在暴露于干扰素诱导剂时培养产生干扰素的细胞，将其暴露于这种诱导物，从其中提取信使RNA 所述诱导细胞，纯化干扰素信使RNA，将信使RNA转录成DNA并将DNA克隆在合适的载体中。 优选的细胞是人二倍体包皮细胞。 本发明还涉及一种用于工程化细菌菌株以产生干扰素多肽的方法，其包括将克隆的干扰素DNA引入合适的载体载体中。 优选的载体载体是大肠杆菌。 本发明还涉及高度纯化形式的人干扰素与β1高度纯化形式的人干扰素的mRNA与（+）2高度纯化形式的人干扰素mRNA相关的编码多肽的DNA 具有干扰素活性，可插入载体，例如质粒pBR322，以及高度纯化形式的人干扰素β1，以及高度纯化形式的人干扰素β2。

    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor
    8.
    发明授权
    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor 失效
    类固醇/甲状腺激素受体相关基因，其在人肝细胞癌中不适当地表达，并且其是视黄酸受体

    公开(公告)号：US5468617A

    公开(公告)日：1995-11-21

    申请号：US190555

    申请日：1994-02-02

    申请人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    发明人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    IPC分类号： C07K14/705 ,  C12Q1/68 ,  G01N33/48

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

    摘要翻译： 将以前分离的乙肝病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp的细胞DNA片段中作为探针，从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。 RAR-β基因由视黄酸（RA）转录上调，其启动子区域可能含有RARE（视黄酸反应元件）。