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1.发明授权Engineering nicking endonucleases from type IIs restriction endonucleases 有权来自II型限制性内切核酸酶的工程化切口内切核酸酶公开(公告)号：US06395523B1
公开(公告)日：2002-05-28
申请号：US09872861
申请日：2001-06-01
申请人： Huimin Kong , Caroline Besnier , Yan Xu
发明人： Huimin Kong , Caroline Besnier , Yan Xu
IPC分类号： C12N900
CPC分类号： C12N9/22
摘要： The present invention relates to methods to engineer nicking endonucleases from existing Type IIs restriction endonucleases, and the production of the engineered nicking endonucleases. Two engineering methods are disclosed. One involves inactivating the dimerization function of a Type IIs restriction enzyme using site-directed mutagenesis approach. The other involves replacing the cleavage domain of a Type IIs restriction enzyme with the cleavage domain from a natural occurring nicking endonuclease, N.BstNBI.
摘要翻译： 本发明涉及从现有的II型限制性内切核酸酶工程化切口内切核酸酶的方法，以及工程化切口内切核酸酶的产生。 公开了两种工程方法。 一个涉及使用定点诱变方法使II型限制酶的二聚功能失活。 另一个涉及用来自天然存在的切口内切核酸酶N.BstNBI的切割结构域替换II型限制酶的切割结构域。
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公开(公告)号：US07829284B2
公开(公告)日：2010-11-09
申请号：US11796675
申请日：2007-04-27
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括使用解旋酶制备物和DNA聚合酶，使得扩增可以等温进行。
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公开(公告)号：US20070207495A1
公开(公告)日：2007-09-06
申请号：US11796675
申请日：2007-04-27
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
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公开(公告)号：US20100075384A1
公开(公告)日：2010-03-25
申请号：US10594095
申请日：2005-03-23
申请人： Huimin Kong , Yan Xu
发明人： Huimin Kong , Yan Xu
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/125 , C12Q2521/513
摘要： A helicase-mediated amplification method for circular DNA templates and target DNA sequences within the templates is provided. The method combines a DNA polymerase and a helicase preparation to amplify a target sequence as well as the entire circular DNA template.
摘要翻译： 提供了解旋酶介导的扩增方法，用于模板内的循环DNA模板和靶DNA序列。 该方法结合DNA聚合酶和解旋酶制剂以扩增靶序列以及整个环状DNA模板。
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公开(公告)号：US07282328B2
公开(公告)日：2007-10-16
申请号：US10665633
申请日：2003-09-19
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括使用解旋酶制备物和DNA聚合酶，使得扩增可以等温进行。
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公开(公告)号：US20060154286A1
公开(公告)日：2006-07-13
申请号：US11327175
申请日：2006-01-05
申请人： Huimin Kong , Myriam Vincent , Yan Xu , Wen Tang
发明人： Huimin Kong , Myriam Vincent , Yan Xu , Wen Tang
CPC分类号： C12P19/34 , C12N15/1096
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括在不存在单链结合蛋白和DNA聚合酶的情况下使用单链解旋酶制剂或热稳定解旋酶，使得可以等温进行扩增。
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公开(公告)号：US07662594B2
公开(公告)日：2010-02-16
申请号：US11327175
申请日：2006-01-05
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12P19/34 , C12N15/1096
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括在不存在单链结合蛋白和DNA聚合酶的情况下使用单链解旋酶制剂或热稳定解旋酶，使得可以等温进行扩增。
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公开(公告)号：US20070254304A1
公开(公告)日：2007-11-01
申请号：US11789553
申请日：2007-04-25
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括使用解旋酶制备物和DNA聚合酶，使得扩增可以等温进行。
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公开(公告)号：US20170285916A1
公开(公告)日：2017-10-05
申请号：US15085677
申请日：2016-03-30
申请人： Yan Xu , Maha El Choubassi , Ronald T. Azuma , Oscar Nestares
发明人： Yan Xu , Maha El Choubassi , Ronald T. Azuma , Oscar Nestares
IPC分类号： G06F3/0484 , G06T11/00 , G06T7/00 , G06T3/00 , G06T5/00
CPC分类号： G06F3/04845 , G06F3/04842 , G06F3/04883 , G06T11/60 , G06T2200/04 , H04N5/2226 , H04N5/2621 , H04N5/272 , H04N5/445
摘要： Embodiments of a system and method for applying camera effects to an image are generally described herein. A method may include receiving, at a device, information including an image and depth data of pixels of the image, generating a set of candidate regions of interest in the image, receiving a user selection of a region of interest of the set of candidate regions of interest, and generating a camera effect for the image using the region of interest and the depth data. The method may include outputting an augmented image, outputting information, outputting a video, or the like.
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