公开(公告)号：US07628961B2

公开(公告)日：2009-12-08

申请号：US10801342

申请日：2004-03-15

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： B01J19/00

CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply heat to, or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以向样品的特定区域提供热量或从其中去除热量，使得通过将相对高的温度区域定位在低于 相对较低的温度区域。

公开(公告)号：US07488595B2

公开(公告)日：2009-02-10

申请号：US10836376

申请日：2004-04-29

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： C12M1/00 ,  C12P19/34

CPC分类号： B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

摘要翻译： 本发明一般涉及使用固定化DNA聚合酶扩增核酸序列的方法和装置。 更具体地说，涉及通过形成能够发生聚合酶链式反应（PCR）的多个反应区域扩增靶核酸序列的方法和装置，将固定的DNA聚合酶定位在特定的反应区域中，并通过反应循环DNA 地区。 本发明提供了在扩增后能够简单地分离和回收DNA聚合酶的方法和装置，其不仅可以用热稳定的DNA聚合酶而且可以用非热稳定的DNA聚合酶来操作，并且它们的设计和过程更简单 使得它们可以容易地集成到诸如片上实验室的复杂器件中。

公开(公告)号：US09346055B2

公开(公告)日：2016-05-24

申请号：US12351764

申请日：2009-01-09

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： B01L7/00

CPC分类号： B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

公开(公告)号：US08053215B2

公开(公告)日：2011-11-08

申请号：US12610245

申请日：2009-10-30

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： C12P19/34 ,  B01J19/00

CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply heat to, or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以向样品的特定区域提供热量或从其中去除热量，使得通过将相对高的温度区域定位在低于 相对较低的温度区域。

公开(公告)号：US20120021463A1

公开(公告)日：2012-01-26

申请号：US13252039

申请日：2011-10-03

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： C12P19/34 ,  C12M1/00

CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以从样品的特定区域供应或除去热量，使得通过将相对高的温度区域定位在比相对低的温度 地区。

公开(公告)号：US20100285536A1

公开(公告)日：2010-11-11

申请号：US12610245

申请日：2009-10-30

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： C12P19/34 ,  C12M1/00 ,  C12M1/02

CPC分类号： C12P19/34 ,  B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

摘要翻译： 本发明提供一种设计简单，易于小型化并且整合到复杂装置中的核酸序列扩增方法及其装置，具有使用不耐热的DNA聚合酶的能力。 在本发明中，组合多个热源以从样品的特定区域供应或除去热量，使得通过将相对高的温度区域定位在比相对低的温度 地区。

公开(公告)号：US20100086975A1

公开(公告)日：2010-04-08

申请号：US12351764

申请日：2009-01-09

申请人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

发明人： Hyun Jin Hwang ,  Jeong Hee Kim ,  Kyunghoon Jeong

IPC分类号： C12P19/34 ,  C12M1/38

CPC分类号： B01L7/525 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0442 ,  B01L2400/0445

摘要： The present invention generally relates to methods and apparatuses for amplifying nucleic acid sequences using immobilized DNA polymerase. More particularly, it relates to methods and apparatuses useful for amplifying target nucleic acid sequences by forming a plurality of reaction regions in which polymerase chain reaction (PCR) can occur, positioning immobilized DNA polymerase in a specific reaction region, and circulating DNA through the reaction regions. The present invention provides those methods and apparatuses that allow simple separation and recovery of the DNA polymerase after the amplification, that can be operated not only with thermostable DNA polymerases but also with non-thermostable DNA polymerases, and that are simpler in their designs and processes so that they can be readily integrated into complex devices such as Lab-on-a-chip.

摘要翻译： 本发明一般涉及使用固定化DNA聚合酶扩增核酸序列的方法和装置。 更具体地说，涉及通过形成能够发生聚合酶链式反应（PCR）的多个反应区域扩增靶核酸序列的方法和装置，将固定的DNA聚合酶定位在特定的反应区域中，并通过反应循环DNA 地区。 本发明提供了在扩增后能够简单地分离和回收DNA聚合酶的方法和装置，其不仅可以用热稳定的DNA聚合酶而且可以用非热稳定的DNA聚合酶来操作，并且它们的设计和过程更简单 使得它们可以容易地集成到诸如片上实验室的复杂器件中。