公开(公告)号：US20080220415A1

公开(公告)日：2008-09-11

申请号：US10593900

申请日：2005-03-25

申请人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

发明人： Han Oh Park ,  Hyun-Bae Kim ,  Sung-Min Chi

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q1/686 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2561/113

摘要： The present invention relates to a detection method of nucleic acid amplification using probe labeled with intercalating dye. More particularly, the present invention is directed to a real-time detection method of nucleic acid amplification, comprising the steps of i) producing an aqueous buffer which contains a nucleic acid, a pair of primers for amplification of said nucleic acid, a fluorescent probe wherein a fluorescent dye of which intensity of fluorescence is varied when the dye is intercalated into a double-stranded nucleic acid, is connected with an oligonucleotide of which base sequence is complementary with at least a part of said nucleic acid, four (4) kinds of nucleotides and DNA polymerase; ii) denaturing said doublestranded nucleic acid into single strands by heating the aqueous buffer prepared in step i) up to 931 C to 96 C; iii) annealing said pair of primers with said single strand by cooling the solution obtained in step ii) up to 50 C. to 571 C; iv) replicating said single-stranded nucleic acid by heating the solution obtained from step iii) up to 701 C to 74° C.; v) denaturing said replicated nucleic add into single strands by heating the solution obtained in step iv) up to 931 C to 961 C; vi) annealing said fluorescent probe with said single-stranded nucleic acid by cooling the solution obtained in step v up to 501 C to 57 C; vii) measuring an intensity of the fluorescence emitted from the solution obtained in step vi); and viii) repeating more than one steps iv) through vii).

摘要翻译： 本发明涉及使用用插层染料标记的探针的核酸扩增检测方法。 更具体地，本发明涉及核酸扩增的实时检测方法，包括以下步骤：i）产生含有核酸的水性缓冲液，用于扩增所述核酸的一对引物，荧光探针 其中当染料插入双链核酸时荧光强度变化的荧光染料与其碱基序列与所述核酸的至少一部分互补的寡核苷酸连接，四（4）种 的核苷酸和DNA聚合酶; ii）通过加热步骤i）中制备的含水缓冲液至931℃至96℃，将所述双链核酸变性为单链; iii）通过将步骤ii）中获得的溶液冷却至50℃至571℃，用所述单链退火所述一对引物; iv）通过将从步骤iii）获得的溶液加热至701℃至74℃来复制所述单链核酸; v）通过将步骤iv）中获得的溶液加热至931℃至961℃，将所述复制的核酸添加到单链中; vi）通过将步骤v中获得的溶液冷却至501℃至57℃，用所述单链核酸退火所述荧光探针; vii）测量从步骤vi）中获得的溶液发射的荧光的强度; 和viii）重复多于一个步骤iv）至vii）。

公开(公告)号：US07220610B2

公开(公告)日：2007-05-22

申请号：US10880099

申请日：2004-06-30

申请人： Hyun-Bae Kim

发明人： Hyun-Bae Kim

IPC分类号： H01L21/00

CPC分类号： G02F1/133553 ,  G02F1/136227

摘要： A manufacturing method of an array substrate for a liquid crystal display device includes forming a switching element in a pixel area on a substrate, wherein the pixel area includes a reflective area and a transmissive area, forming an organic layer on an entire surface of the substrate including the switching element, first exposing the organic layer using a first mask, second exposing the first exposed organic layer using a second mask, developing the first and second exposed organic layer to form a passivation layer having an unevenness pattern in the reflective area, forming a reflector on the passivation layer in the reflective area, and forming a pixel electrode on the reflector in the reflective area and the transmissive area.

摘要翻译： 一种液晶显示装置用阵列基板的制造方法，其特征在于，在基板的像素区域中形成开关元件，所述像素区域包括反射区域和透射区域，在所述基板的整个表面上形成有机层 包括所述开关元件，首先使用第一掩模曝光所述有机层，使用第二掩模将所述第一暴露的有机层第二曝光，使所述第一和第二暴露的有机层显影，以形成在所述反射区域中具有不平坦图案的钝化层，形成 在反射区域中的钝化层上的反射器，以及在反射区域和透射区域中的反射器上形成像素电极。

公开(公告)号：US20050142678A1

公开(公告)日：2005-06-30

申请号：US10880099

申请日：2004-06-30

申请人： Hyun-Bae Kim

发明人： Hyun-Bae Kim

IPC分类号： G02F1/136 ,  G02F1/1335 ,  G02F1/1362

CPC分类号： G02F1/133553 ,  G02F1/136227

摘要： A manufacturing method of an array substrate for a liquid crystal display device includes forming a switching element in a pixel area on a substrate, wherein the pixel area includes a reflective area and a transmissive area, forming an organic layer on an entire surface of the substrate including the switching element, first exposing the organic layer using a first mask, second exposing the first exposed organic layer using a second mask, developing the first and second exposed organic layer to form a passivation layer having an unevenness pattern in the reflective area, forming a reflector on the passivation layer in the reflective area, and forming a pixel electrode on the reflector in the reflective area and the transmissive area.

摘要翻译： 一种液晶显示装置用阵列基板的制造方法，其特征在于，在基板的像素区域中形成开关元件，所述像素区域包括反射区域和透射区域，在所述基板的整个表面上形成有机层 包括所述开关元件，首先使用第一掩模曝光所述有机层，使用第二掩模将所述第一暴露的有机层第二曝光，使所述第一和第二暴露的有机层显影，以形成在所述反射区域中具有不平坦图案的钝化层，形成 在反射区域中的钝化层上的反射器，以及在反射区域和透射区域中的反射器上形成像素电极。

公开(公告)号：US06667792B2

公开(公告)日：2003-12-23

申请号：US10029175

申请日：2001-12-28

申请人： Dug-Jin Park ,  Seong-Hee Kim ,  Hyun-Bae Kim

发明人： Dug-Jin Park ,  Seong-Hee Kim ,  Hyun-Bae Kim

IPC分类号： G02F1136

CPC分类号： G02F1/1333 ,  G02F2001/133374

摘要： An array substrate for liquid crystal display devices includes a transparent substrate, a plurality of array cells on the transparent substrate, each of the array cells include a display area and a non-display area having a first identification, and a semiconductor layer including a second identification mark.