Abstract:
The invention relates to a process for treating a lignocellulosic biomass comprising a solids content of not more than 80% by weight, said process comprising the use of at least one reactor (9,14) for treating said biomass, in which the or at least one of said reactors is fed with biomass via a feed means (6,11) creating a pressure increase between the biomass inlet and the biomass outlet of said feed means, in which said feed means is washed by circulation of a washing fluid between a washing inlet (7,12) and a washing outlet (8,13). According to the process, at least a portion of the washing fluid (8,13) exiting the fluid outlet of the at least one feed means (6,11) is reintroduced into the washing inlet of the same feed means or of another of said feed means.
Abstract:
A process for producing oligosaccharides from lignocellulosic biomass, having the following steps: a) pre-treating the biomass in a pre-treatment reactor (1) in order to provide an effluent containing a pre-treated substrate; b) enzymatic hydrolysis of the pre-treated substrate contained in the effluent from step a) in a reactor, in the presence of cellulases such that a hydrolysate containing glucose, cellulases and water is produced; c) removing at least a portion of the hydrolysate from step b) comprising a liquid fraction; d) reducing the water content of said portion of the hydrolysate in step c) such that the liquid fraction of the hydrolysate has a water content of less than 65% by weight with respect to the total weight of the liquid fraction; e) incubating the hydrolysate from step d) at a temperature in the range 40° C. to 70° C. for the time necessary to produce an effluent enriched in oligosaccharides.
Abstract:
The present invention relates to a process for treating lignocellulosic biomass, successively comprising b) a step of pretreatment of the biomass placed beforehand under acidic or neutral pH conditions in a pretreatment reactor (3), to produce an acidic or neutral pretreated must (AM), alternating with b′) a step of pretreatment of the biomass placed beforehand under acidic, neutral or basic conditions, with optional sufficient introduction of a basic aqueous solution (EB) into the pretreatment reactor (3) to produce a basic pretreated must (BM), and then c) a step of enzymatic hydrolysis in a hydrolysis reactor (16) of a mixture of the acidic or neutral pretreated must (AM) obtained from step b) with the basic pretreated must (BM) obtained from step b′).
Abstract:
The invention relates to a process for treating lignocellulosic biomass by means of an acidic liquor in order to produce “second-generation” (2G) sugary liquors. These sugary liquors may be used to produce other products via a biochemical pathway (e.g. alcohols such as ethanol, butanol or other molecules, for example solvents such as acetone, etc.). This process comprises analysis of the liquid streams withdrawn during the process and adjustment of the composition of the impregnation liquor so as to keep the acidic power of said streams constant.
Abstract:
The present invention concerns a process for the production of an aqueous mixture comprising isopropanol, n-butanol and ethanol, comprising a step in which an aqueous solution containing C5 and/or C6 sugars and acetate is fermented under strict anaerobic conditions in the presence of a fermenting microorganism of the genus Clostridium and in which said aqueous solution has an acetate concentration in the range 0.5 to 10 g/L and a ratio by weight of (acetate)/(sum of C5 and/or C6 sugars) in the range 0.005 to 0.35 g/g.
Abstract:
The process for the production of alcohol and/or solvent from a biomass feedstock comprises the stages for pretreatment (P) of the biomass feedstock, for enzymatic hydrolysis (H) of the pretreated substrate, and for fermenting the hydrolyzate (F).To reduce the size of the fermenters, at least a portion of the solid residue contained in the hydrolyzate is extracted (Ex1) in such a way as to obtain a stream of solid residue (9) comprising lignin and a hydrolyzate (8) that is low in solid residue. Then, the stream of solid residue is washed (L) with a liquid stream in such a way as to recover a sugar-enriched liquid stream (15). The sugar-enriched liquid stream (15) is recycled in the enzymatic hydrolysis stage (H) to be able to upgrade the sugars without providing dilution of the streams in the process.
Abstract:
The present invention relates to a process for treating a lignocellulosic biomass pretreated beforehand (1), said process comprising the extraction of the free sugars from said pretreated biomass using an alcoholic or aqueous-alcoholic extraction solution (2), so as to obtain a liquid phase enriched in free sugars, called liquor (3), and a solid phase depleted in free sugars, called must (4).
Abstract:
The present invention relates to a processing method at acidic or neutral pH in a reactor (4) for processing lignocellulosic biomass (P), said process including a continuous cleaning phase of the reactor which comprises introducing a basic aqueous solution (EB) into said reactor containing the biomass being processed.
Abstract:
The present invention concerns a method for cleaning a reactor (4) for treating a lignocellulosic biomass (P), said method comprising the following steps: a) draining the reactor of the reaction mixture containing the biomass, b) filling the reactor with a basic aqueous solution (EB), c) draining the reactor, d) injecting steam (V) into the reactor, e) filling the reactor with an aqueous solution (E), f) draining the reactor.
Abstract:
The present invention relates to a process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi, said process comprising the following steps: (a) a first step of growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor, (b) a second step of producing enzymes, starting with the aqueous culture medium obtained in the first step (a), in the presence of at least one inductive carbon-based substrate, also inducing the production of hydrophobins, and, in a step (d), at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).