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    Transposon end compositions and methods for modifying nucleic acids
    3.
    发明授权
    Transposon end compositions and methods for modifying nucleic acids 有权

    公开(公告)号：US11118175B2

    公开(公告)日：2021-09-14

    申请号：US16210284

    申请日：2018-12-05

    Applicant: Illumina, Inc.

    Inventor： Haiying Li Grunenwald ,  Nicholas Caruccio ,  Jerome Jendrisak ,  Gary Dahl

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6869

    Abstract: The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing so-called “next generation sequencing”).

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