公开(公告)号：US20240287583A1

公开(公告)日：2024-08-29

申请号：US18571069

申请日：2023-04-06

申请人： ILLUMINA, INC.

发明人： Andrew Slatter ,  Carlo Randise-Hinchliff ,  Andrew Price ,  Niall Anthony Gormley ,  Andrea Manzo ,  Nithya Subramanian ,  Fiona Kaper ,  David Jones ,  Steven Norberg

IPC分类号： C12Q1/682 ,  C12Q1/6839

CPC分类号： C12Q1/682 ,  C12Q1/6839

摘要： Aptamer detection techniques with dynamic range compression are described that permit removal of a portion of more abundant aptamers in an aptamer-based assay. In an embodiment, a mixture of tagged probes and dummy probes can be used such that the dummy probes bind abundant aptamers and in turn are not captured or amplified for detection in downstream steps. Other techniques are also contemplated, including targeted removal of or cleavage of probes that bind to excess aptamers.