公开(公告)号：US12186746B2

公开(公告)日：2025-01-07

申请号：US17255819

申请日：2020-02-07

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

公开(公告)号：US20250108375A1

公开(公告)日：2025-04-03

申请号：US18979010

申请日：2024-12-12

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

公开(公告)号：US20210362151A1

公开(公告)日：2021-11-25

申请号：US17255819

申请日：2020-02-07

Applicant: ILLUMINA, INC.

Inventor： Aathavan Karunakaran ,  Arnaud Rival ,  Ali Agah ,  Fabien Abeille ,  Steven Barnard ,  Craig Ciesla ,  Murali Venkatesan ,  Pargol Gheissari ,  Jennifer Wang ,  Dietrich Dehlinger

IPC: B01L3/00

Abstract: A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.