公开(公告)号：US20240301487A1

公开(公告)日：2024-09-12

申请号：US18600605

申请日：2024-03-08

Applicant: ILLUMINA, INC.

Inventor： Samantha Kelly Brittelle ,  Tyler J. Dill ,  Michelle Kate Fu ,  Ryan David McCurdy ,  Michael L. Neville ,  Sean M. Ramirez ,  Stuart A. Sardo

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806

Abstract: In an example method, a grafting solution is applied to a patterned substrate using a liquid-phase thin-film deposition technique. The patterned substrate includes a lane surrounded by, or a plurality of depressions separated by interstitial regions; and a polymer in the lane or in each of the plurality of depressions. The polymer is functionalized with a first click reaction moiety. The grafting solution includes a solvent; a polymer matrix material dissolved in the solvent; and primers of a primer set dissolved in the solvent, each of the primers being terminated with a second click reaction moiety. The applied grafting solution is dried. During drying, a solid polymer matrix is formed and at least some of the primers attach to the polymer i) via the first and second click reaction moieties and ii) in at least a portion of the lane or in at least some of the plurality of depressions.