公开(公告)号：US20250019758A1

公开(公告)日：2025-01-16

申请号：US18650800

申请日：2024-04-30

Applicant: ILLUMINA, INC.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Tsz Ying Yuen ,  Daniel Hartoyo Lukamto ,  Erin Garcia ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods for modification-based controlled polynucleotide translocation in nanopores for sequencing, modified nucleotides, and kits and systems for performing the disclosed methods. In some embodiments, modifications can be used to control polynucleotide translocation by modifying nucleotides on a strand of polynucleotide to carry a modification, where the modifications can arrest or slow translocation when encountering the nanopore. In some embodiments, application of a voltage can move one nucleotide and its attached modification through the nanopore at a time.