公开(公告)号：US20230357307A1

公开(公告)日：2023-11-09

申请号：US18311876

申请日：2023-05-03

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE LTD.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Min Yen Lee ,  Ramesh Neelakandan ,  Jeffrey G. Mandell ,  Erin Garcia ,  Sharyuen Soh ,  Daniel Hartoyo Lukamto

IPC: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

CPC classification number: C07H21/00 ,  C12Q1/6869 ,  C07H19/10

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a linker construct between two positions of the nucleotide, wherein the linker construct optionally comprises a reporter moiety corresponding to the identity of the nucleotide, and wherein the linker construct is a part of the cleavable cyclic loop nucleotide comprising a cleavable site. In some embodiments, the nucleotides further comprise arresting constructs for slowing or halting the polynucleotide translocation through a nanopore.

公开(公告)号：US20250109433A1

公开(公告)日：2025-04-03

申请号：US18887792

申请日：2024-09-17

Applicant: Illumina, Inc.

Inventor： Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Ramesh Neelakandan ,  Erin Garcia ,  Abdul Sadeer Abd Salam ,  Daniel Hartoyo Lukamto ,  Soumadwip Ghosh ,  Xiangyuan Yang ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6853

Abstract: In one aspect, the disclosed technology relates to nanopore sequencing with a polynucleotide comprising a plurality of nucleotides, wherein each nucleotide comprises a macromolecular block. In some embodiments, the macromolecular blocks are configured for slowing or halting the polynucleotide translocation through a nanopore. In some embodiments the macromolecular blocks have linear and branched structures.

公开(公告)号：US20250019758A1

公开(公告)日：2025-01-16

申请号：US18650800

申请日：2024-04-30

Applicant: ILLUMINA, INC.

Inventor： Abdul Sadeer Abd Salam ,  Xiangyuan Yang ,  Hassan Zakiruddin Bohra ,  Yin Nah Teo ,  Tsz Ying Yuen ,  Daniel Hartoyo Lukamto ,  Erin Garcia ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods for modification-based controlled polynucleotide translocation in nanopores for sequencing, modified nucleotides, and kits and systems for performing the disclosed methods. In some embodiments, modifications can be used to control polynucleotide translocation by modifying nucleotides on a strand of polynucleotide to carry a modification, where the modifications can arrest or slow translocation when encountering the nanopore. In some embodiments, application of a voltage can move one nucleotide and its attached modification through the nanopore at a time.

公开(公告)号：US20250109434A1

公开(公告)日：2025-04-03

申请号：US18900392

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Hugo Brandão ,  John Moon ,  Jeffrey G. Mandell ,  Erin Garcia ,  Lisa Savagian ,  Eunho Noh ,  Xiangyuan Yang ,  Sharyuen Soh ,  Sadeer Salam

IPC: C12Q1/6869 ,  C12Q1/6809

Abstract: A polymer is disposed through a nanopore that encodes a polynucleotide's sequence and includes monomer units that encode nucleotides and include first and second reporter moieties and first and second arresting constructs. The first reporter moiety is translocated into the nanopore's aperture, where a first value of an electrical property of the first reporter moiety is measured while the first arresting construct pauses translocation. The second reporter moiety is translocated into the aperture, where a second value of an electrical property of the second reporter moiety is measured while the second arresting construct pauses translocation. The first value and the second value for each monomer unit is used to identify the nucleotide encoded by that monomer unit; and distinguish the nucleotide encoded by that monomer unit from the nucleotides encoded by adjacent monomer units, including by those that encode the same type of nucleotide as that monomer unit.