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公开(公告)号：US20180345287A1
公开(公告)日：2018-12-06
申请号：US16059576
申请日：2018-08-09
Applicant: IMEC VZW
Inventor： Paolo Fiorini , Tim Stakenborg , Frederik Colle
CPC classification number: B01L3/502784 , B01L7/52 , B01L2200/061 , B01L2200/0673 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2300/0883 , B01L2300/1827 , C12Q1/686
Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
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公开(公告)号：US11266990B2
公开(公告)日：2022-03-08
申请号：US16059576
申请日：2018-08-09
Applicant: IMEC VZW
Inventor： Paolo Fiorini , Tim Stakenborg , Frederik Colle
Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
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公开(公告)号：US20150151301A1
公开(公告)日：2015-06-04
申请号：US14547737
申请日：2014-11-19
Applicant: IMEC VZW
Inventor： Paolo Fiorini , Tim Stakenborg , Frederik Colle
CPC classification number: B01L3/502784 , B01L7/52 , B01L2200/061 , B01L2200/0673 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/0867 , B01L2300/0883 , B01L2300/1827 , C12Q1/686
Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
Abstract translation: 提出了一种用于执行数字PCR的微流体装置100。 该器件包括：半导体衬底; 第一微流体通道104，包括嵌入在半导体衬底中的入口102和出口103; 热耦合到第一微流体通道104的加热元件101; 液滴发生器107，其连接到第一微流体通道104的入口102，用于产生液滴并以流速泵送产生的液滴到第一微流体通道104中; 其特征在于：加热元件101是连接到温度控制单元111的单个加热元件，温度控制单元111被配置为使完整的第一微流体通道104的温度循环至少两个温度值; 并且其中液滴发生器107的流量适应。 此外，使用微流体装置100呈现执行数字PCR的方法。
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