公开(公告)号：US20220305113A1

公开(公告)日：2022-09-29

申请号：US17827256

申请日：2022-05-27

Applicant: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES

Inventor： Lianpan DAI ,  Jinghua YAN ,  Fu GAO ,  Yan LI ,  Tianyi ZHENG ,  Kun XU ,  Mei LIU ,  Yaling AN ,  Yi SHI

IPC: A61K39/215 ,  C07K14/005 ,  A61K39/39 ,  A61P31/14

Abstract: The embodiments of the present disclosure relate to antigens of β-coronaviruses, preparation methods and uses thereof. The amino acid sequence of the antigen of the β-coronavirus includes an amino acid sequence arranged in a (A-B)-(A-B) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B) pattern or an amino acid sequence arranged in a (A-B)-(A-B′) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B′) pattern. The antigen of the β-coronavirus has a single-chain dimer structure. A single-chain dirtier expressed according to examples of the present disclosure is stable in content and has excellent immunogenicity as an antigen of a β-coronavirus, and a vaccine prepared by using the single-chain dimer as an antigen of a β-coronavirus can elicit high-titer neutralizing antibodies in mice.