公开(公告)号：US11549140B2

公开(公告)日：2023-01-10

申请号：US17052519

申请日：2019-05-02

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12P19/34 ,  G01N33/487 ,  H01P7/00 ,  H03H7/06

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US10995373B2

公开(公告)日：2021-05-04

申请号：US16836689

申请日：2020-03-31

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12P19/34 ,  G01N33/487 ,  H01P7/00 ,  H03H7/06

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US10714178B2

公开(公告)日：2020-07-14

申请号：US16514726

申请日：2019-07-17

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： G11C13/02 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B30/00 ,  G06N3/12 ,  B82Y10/00

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive variance as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US11505825B2

公开(公告)日：2022-11-22

申请号：US17233186

申请日：2021-04-16

申请人： IRIDIA, INC.

发明人： Paul F. Predki

IPC分类号： C12P19/34 ,  C12Q1/6869 ,  G01N33/487 ,  H01P7/00 ,  H03H7/06

摘要： The disclosure provides methods of synthesizing DNA using topoisomerase-mediated ligation, by adding single nucleotides or oligomers to a DNA strand in the 3′ to 5′ direction.

公开(公告)号：US10859562B2

公开(公告)日：2020-12-08

申请号：US16399746

申请日：2019-04-30

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： G01N33/48 ,  G01N33/487 ,  G11C11/4074 ,  C07H21/04 ,  C12Q1/6869 ,  G16B30/00 ,  B82Y10/00 ,  C12P19/34

摘要： Methods, systems and devices for reading data stored in a polymer (e.g., DNA) and for verifying the sequence of a polymer synthesized in situ in a nanopore-based chip, include providing a resonator having an inductor and a cell, the cell having a nanopore and a polymer that can traverse through the nanopore, the resonator having an AC output voltage frequency response at a probe frequency in response to an AC input voltage at the probe frequency, providing the AC input voltage having at least the probe frequency, and monitoring the AC output voltage at least at the probe frequency, the AC output voltage at the probe frequency being indicative of the data stored in the polymer at the time of monitoring, wherein the polymer includes at least two monomers having different properties causing different resonant frequency responses.

公开(公告)号：US20190136307A1

公开(公告)日：2019-05-09

申请号：US15969745

申请日：2018-05-02

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： C12Q1/6869 ,  C12P19/34 ,  G01N33/487

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US11545213B1

公开(公告)日：2023-01-03

申请号：US17396439

申请日：2021-08-06

申请人： IRIDIA, INC.

发明人： Paul F. Predki

IPC分类号： G11C13/00 ,  G11C11/56 ,  H01L51/05 ,  H01L51/00 ,  H01L27/28

摘要： The disclosure provides a novel system and method of storing multi-bit information, including providing a nano-channel-based polymer memory device, the device having at least one memory cell comprising at least two addition nano-channels, each of the addition nano-channels arranged to add a unique chemical construct (or codes) to the polymer when the polymer enters the respective addition nano-channel, the polymer having a bead or origami on a non-writing end of the polymer; each nano-channel having a nano-port constriction having a port width which allows the polymer to pass through the nano-port, and does not allow the bead or origami to pass through and does not allow addition or deblocking enzymes (or beads attached thereto) to pass through the nano-port; successively steering the polymer through the nanopore into the addition nano-channels to add the codes to the polymer based on a predetermined digital data pattern to create the digital data pattern on the polymer.

公开(公告)号：US11152061B2

公开(公告)日：2021-10-19

申请号：US16866364

申请日：2020-05-04

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  John Stuart Foster

IPC分类号： G11C13/00 ,  G05B11/42

摘要： A system and method of storing and reading digital data, including providing a nanopore polymer memory (NPM) device having at least one memory cell comprising at least two addition chambers each arranged to add a unique chemical construct (or codes) to a polymer (or DNA) string when the polymer enters the respective addition chamber, the data comprising a series of codes; successively steering the polymer from deblock chambers through the nanopore into the addition chambers to add codes to the polymer to create the digital data pattern on the polymer; and accurately controlling the bit rate of the polymer using a servo controller. The device may have loading chamber(s) to load (or remove) the polymer into/from the deblock chambers through at least one “micro-hole”. The cell may be part of a memory system that stores and retrieves “raw” data and allows for remote retrieval and conversion. The cell may store multi-bit data having a plurality of states for the codes.

公开(公告)号：US20200224264A1

公开(公告)日：2020-07-16

申请号：US16836689

申请日：2020-03-31

申请人： IRIDIA, INC.

发明人： Paul F. Predki ,  Maja Cassidy

IPC分类号： C12Q1/6869 ,  C12P19/34 ,  G01N33/487

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive or impedance variance, e.g., via a change in a resonant frequency response, as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US10438662B2

公开(公告)日：2019-10-08

申请号：US15690189

申请日：2017-08-29

申请人： IRIDIA, INC.

发明人： Paul F. Predki

IPC分类号： G11C13/02 ,  C12Q1/68 ,  C12Q1/6869 ,  G16B30/00 ,  G06N3/12 ,  B82Y10/00

摘要： The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive variance as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.