公开(公告)号：US20030139885A1

公开(公告)日：2003-07-24

申请号：US10289462

申请日：2002-11-05

Applicant: IRM, LLC

Inventor： Ansgar Brock ,  David M. Horn ,  Eric C. Peters

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N33/6848 ,  B82Y30/00 ,  G01N33/6818 ,  G01N33/6842 ,  G01N33/6851 ,  G01N2035/00158 ,  G01N2458/15 ,  G16B30/00 ,  G16B50/00 ,  H01J49/0036

Abstract: Provided are methods and systems for identification of proteins using high mass accuracy mass spectrometry. Not only do high mass accuracy measurements provide greater confidence in protein identification assignments, but they also enable proteins to be identified with either less sequence coverage or fewer additional tandem MS experiments. In addition, high mass measurement accuracy optionally allows protein identifications to be made on the basis of the mass of a single peptide, providing higher-throughputs in the analysis of mixtures due to the significant decrease in time spent on additional tandem MS experiments. In addition, a concomitant time saving in the cross correlation process of mass spectral data with in silico digested databases would also be achieved.

Abstract translation: 提供了使用高质量精度质谱法鉴定蛋白质的方法和系统。 不仅高质量精度测量对蛋白质鉴定分配提供了更大的信心，而且还使蛋白质能够以更少的序列覆盖率或更少的附加串联MS实验来鉴定。 此外，高质量测量精度可选地允许基于单个肽的质量进行蛋白质鉴定，由于在额外的串联MS实验中花费的时间的显着减少，在混合物的分析中提供更高的通量。 此外，也可以实现在计算机数据库中同时节省质谱数据的互相关过程。