公开(公告)号：US10233493B2

公开(公告)日：2019-03-19

申请号：US15853118

申请日：2017-12-22

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

公开(公告)号：US09862998B2

公开(公告)日：2018-01-09

申请号：US15132662

申请日：2016-04-19

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2525/101 ,  C12Q2565/1015

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

公开(公告)号：US20180155783A1

公开(公告)日：2018-06-07

申请号：US15853118

申请日：2017-12-22

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2525/101 ,  C12Q2565/1015

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

公开(公告)号：US20230235395A1

公开(公告)日：2023-07-27

申请号：US18129747

申请日：2023-03-31

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

公开(公告)号：US11643684B2

公开(公告)日：2023-05-09

申请号：US17091845

申请日：2020-11-06

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6874 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2525/101 ,  C12Q1/6874 ,  C12Q2565/133 ,  C12Q2563/125 ,  C12Q2565/1015

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

公开(公告)号：US20210164040A1

公开(公告)日：2021-06-03

申请号：US17091845

申请日：2020-11-06

申请人： Illumina, Inc.

发明人： Molly He ,  Cheng-Yao Chen ,  Eric Kool ,  Mostafa Ronaghi ,  Michael Previte ,  Rigo Pantoja

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6818

摘要： This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.