公开(公告)号：US20230383284A1

公开(公告)日：2023-11-30

申请号：US18447800

申请日：2023-08-10

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Maria Candelaria Rogert BACIGALUPO ,  Frank STEEMERS ,  Jeffrey FISHER ,  Andrew SLATTER ,  Lewis KRAFT ,  Niall GORMLEY ,  M. Shane BOWEN

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1093

摘要： Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.

公开(公告)号：US20220186307A1

公开(公告)日：2022-06-16

申请号：US17643454

申请日：2021-12-09

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Lewis KRAFT ,  Craig Lee HETHERINGTON ,  Craig M. CIESLA ,  Michael BUREK ,  Jeffrey FISHER ,  Jason BETLEY

IPC分类号： C12Q1/6869 ,  H01L27/146

摘要： An image sensor structure includes an image layer having an array of light detectors disposed therein. A device stack is disposed over the image layer. An array of light guides is disposed in the device stack. Each light guide is associated with a light detector. An array of nanowells is disposed over the device stack. Each nanowell is associated with a first light guide of the array of light guides. A first primer set is disposed throughout a first well region of each nanowell. A second primer set is disposed throughout a second well region of each nanowell. The second well region is adjacent the first well region. The first and second primer sets are operable to attach a forward strand cluster of forward polynucleotide strands in the first well region and a reverse strand cluster of reverse polynucleotide strands in the second well region.

公开(公告)号：US20220002711A1

公开(公告)日：2022-01-06

申请号：US17358892

申请日：2021-06-25

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Maria Candelaria Rogert BACIGALUPO ,  Frank STEEMERS ,  Jeffrey FISHER ,  Andrew SLATTER ,  Lewis KRAFT ,  Niall GORMLEY ,  M. Shane BOWEN

IPC分类号： C12N15/10

摘要： Provided is a method, including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments. Also provided is a method including stretching a polynucleotide over a substrate including a plurality of equally spaced cleavage regions including a plurality of transposases, cleaving the polynucleotide with two or more of the plurality of transposases to form a plurality of polynucleotide fragments, and separating, within the plurality of polynucleotide fragments, a population of longer polynucleotide fragments from a population of shorter polynucleotide fragments.