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公开(公告)号：US20230303970A1
公开(公告)日：2023-09-28
申请号：US18134451
申请日：2023-04-13
申请人： Inguran, LLC
CPC分类号： C12N5/061 , G01N33/582 , C12N13/00 , G01N1/30
摘要： The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
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公开(公告)号：US20180208894A1
公开(公告)日：2018-07-26
申请号：US15875679
申请日：2018-01-19
申请人： Inguran, LLC
CPC分类号： C12N5/0612 , A01N1/0205 , A01N1/0215 , A01N1/0221 , A01N1/0226
摘要： Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.
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公开(公告)号：US11279913B2
公开(公告)日：2022-03-22
申请号：US15875679
申请日：2018-01-19
申请人： Inguran, LLC
摘要： Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.
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4.发明申请SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE AND METHODS OF PRODUCING SEX SORTED SPERM DEMONSTRATING A DOSE RESPONSE 审中-公开性别分散的化学成分反应和产生代谢分子的化学反应的方法公开(公告)号：US20160010057A1
公开(公告)日：2016-01-14
申请号：US14861572
申请日：2015-09-22
申请人： Inguran, LLC
发明人： Ramakrishnan Vishwanath , Kenneth Michael Evans , Thomas B. Gilligan , Juan Moreno , Richard Lenz , Clara Gonzalez-Marin
CPC分类号： C12N5/0612 , C12N5/061 , G01N1/30 , G01N15/1425 , G01N15/1459 , G01N33/5005 , G01N33/52 , G01N2015/1006 , G01N2015/149
摘要： Embodiments of the claimed invention relate to a method of producing sex sorted sperm having an improved dose response which includes the steps of extending a sperm sample with a buffering holding media and adjusting the concentration of the extended sperm sample to a target concentration range. The extended sperm may then be stained with a DNA selective dye and sex sorted with a flow cytometer into a catch fluid. During the extending and sorting of the sperm sample, the pH may be maintained at a target pH. Additionally, at least one of the buffering holding media, the DNA selective dye and the catch fluid may be supplemented with at least one antioxidant.
摘要翻译： 要求保护的发明的实施方案涉及产生具有改善的剂量反应的性别分类精子的方法，其包括以下步骤：用缓冲保持培养基延长精子样品并将扩展的精子样品的浓度调整到目标浓度范围。 然后将扩展的精子用DNA选择性染色剂染色并用流式细胞仪分选成捕获液。 在精子样品的延伸和分选过程中，pH可以保持在目标pH。 此外，至少一种缓冲保持介质，DNA选择性染料和捕获流体可以补充至少一种抗氧化剂。
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公开(公告)号：US20140234833A1
公开(公告)日：2014-08-21
申请号：US14348194
申请日：2012-09-28
申请人： Inguran LLC
CPC分类号： G01N33/5005 , C12N5/061 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
摘要翻译： 公开了一种性别分类精子的方法。 精子可能被DNA选择性荧光染料染色，当激发时发出荧光，残留的淬灭染料会选择性地猝灭受精精子膜内的DNA选择性荧光染料发出的荧光，以及分裂增强染料。 然后将染色的精子分选成一个或多个性别富集的活的精子亚群。
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公开(公告)号：US20130084558A1
公开(公告)日：2013-04-04
申请号：US13631249
申请日：2012-09-28
申请人： INGURAN, LLC
CPC分类号： G01N33/5005 , C12N5/061 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
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公开(公告)号：US20220186182A1
公开(公告)日：2022-06-16
申请号：US17666325
申请日：2022-02-07
申请人： Inguran, LLC
摘要： Embodiments of the present invention relate generally to processes, systems, and compositions useful in manipulating a ratio of viable X chromosome bearing sperm to viable Y chromosome bearing sperm in at least one sperm population and useful for preserving the resulting manipulated sperm population. In some embodiments a cryoprotectant may be incorporated into various medias used in manipulating the sperm sample, such as in a staining media, a sheath fluid, and a collection media.
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公开(公告)号：US20200299640A1
公开(公告)日：2020-09-24
申请号：US16823001
申请日：2020-03-18
申请人： Inguran, LLC
摘要： The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
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公开(公告)号：US09581587B2
公开(公告)日：2017-02-28
申请号：US14348194
申请日：2012-09-28
申请人： INGURAN, LLC
CPC分类号： G01N33/5005 , C12N5/061 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.
摘要翻译： 公开了一种性别分类精子的方法。 精子可能被DNA选择性荧光染料染色，当激发时发出荧光，残留的淬灭染料会选择性地猝灭受精精子膜内的DNA选择性荧光染料发出的荧光，以及分裂增强染料。 然后将染色的精子分选成一个或多个性别富集的活的精子亚群。
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公开(公告)号：US20140099627A1
公开(公告)日：2014-04-10
申请号：US13784578
申请日：2013-03-04
申请人： INGURAN, LLC
发明人： Thomas Boyd Gilligan , Kenneth Michael Evans , Richard Lenz , Clara Gonzalez-Marin , Ramakrishnan Vishwanath
IPC分类号： C12N5/076
摘要： Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.
摘要翻译： 公开了处理和分选精子的方法。 精子分选或染色过程的一部分可以包括通过将精子样品的浓度调节到预定浓度并将样品的pH调节到预定值来标准化精子样品。 精子也可以在具有DNA选择性染料和淬灭染料的单一染色缓冲液中染色。
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