公开(公告)号：USD784555S1

公开(公告)日：2017-04-18

申请号：US29507799

申请日：2014-10-30

申请人： Inguran, LLC

设计人： Thomas Boyd Gilligan ,  Sam Hogue

公开(公告)号：US20140099628A1

公开(公告)日：2014-04-10

申请号：US14045617

申请日：2013-10-03

申请人： INGURAN, LLC

发明人： Kenneth Michael Evans ,  Thomas Boyd Gilligan ,  Richard W. Lenz ,  Ramakrishnan Vishwanath

IPC分类号： G01N33/58

CPC分类号： G01N33/582 ,  C12N5/061 ,  C12N5/0612 ,  G01N15/1425 ,  G01N15/1459 ,  G01N33/5005 ,  G01N33/52 ,  G01N2015/1006 ,  G01N2015/1415 ,  G01N2015/149

摘要： Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

摘要翻译： 细胞分选方法，通过提高分选压力并结合某些步骤来提高分选效率和生产率，以帮助细胞更好地经受这种升高的压力。 在精子的情况下，分选标准化精子样本的步骤，在一个步骤中染色精子样品，校准流式细胞计数器将精子置于液滴的前缘，并且改变捕获流体距离可单独或组合 帮助精子更好地生存在性别分类过程中。

公开(公告)号：US11448653B2

公开(公告)日：2022-09-20

申请号：US16806853

申请日：2020-03-02

申请人： Inguran, LLC

发明人： Kenneth Michael Evans ,  Thomas Boyd Gilligan ,  Richard W. Lenz ,  Ramakrishnan Vishwanath ,  Clara Gonzalez-Marin

IPC分类号： G01N33/58 ,  G01N33/50 ,  G01N15/14 ,  G01N33/52 ,  C12N5/071 ,  C12N5/076 ,  G01N15/10

摘要： Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

公开(公告)号：US20140099664A1

公开(公告)日：2014-04-10

申请号：US13784597

申请日：2013-03-04

申请人： INGURAN, LLC

发明人： Kenneth Michael Evans ,  Thomas Boyd Gilligan ,  Johnathan Charles Sharpe ,  Juan Moreno ,  Ramakrishnan Vishwanath

IPC分类号： C12N5/071

CPC分类号： C12N5/061 ,  C12N5/0612 ,  C12Q1/6806 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2563/173

摘要： This disclosure relates to cell sorting methods, and particularly cell sorting methods that improve the efficiency or productivity of sorting in a particle sorting instrument utilizing a measured parameter of sorting efficiency. In one embodiment, minimum productivity and minimum purity may be established and maintained while attempting to maximize the sorting efficiency. While in another embodiment, a minimum sorting efficiency and a minimum purity may be established and maintained while attempting to maximize the productivity of a sort.

摘要翻译： 本公开涉及细胞分选方法，特别是细胞分选方法，其利用测定的分选效率参数提高粒子分选仪器中的分选效率或生产率。 在一个实施方案中，可以在尝试使分选效率最大化的同时建立和维持最低生产率和最低纯度。 而在另一个实施方案中，可以在尝试最大化一种生产率的同时建立和维持最小分选效率和最小纯度。

公开(公告)号：US10400213B2

公开(公告)日：2019-09-03

申请号：US13784597

申请日：2013-03-04

申请人： INGURAN, LLC

发明人： Kenneth Michael Evans ,  Thomas Boyd Gilligan ,  Johnathan Charles Sharpe ,  Juan Moreno ,  Ramakrishnan Vishwanath

IPC分类号： C12N5/071 ,  C12N5/076 ,  C12Q1/6806

摘要： This disclosure relates to cell sorting methods, and particularly cell sorting methods that improve the efficiency or productivity of sorting in a particle sorting instrument utilizing a measured parameter of sorting efficiency. In one embodiment, minimum productivity and minimum purity may be established and maintained while attempting to maximize the sorting efficiency. While in another embodiment, a minimum sorting efficiency and a minimum purity may be established and maintained while attempting to maximize the productivity of a sort.

公开(公告)号：US20140099627A1

公开(公告)日：2014-04-10

申请号：US13784578

申请日：2013-03-04

申请人： INGURAN, LLC

发明人： Thomas Boyd Gilligan ,  Kenneth Michael Evans ,  Richard Lenz ,  Clara Gonzalez-Marin ,  Ramakrishnan Vishwanath

IPC分类号： C12N5/076

摘要： Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.

摘要翻译： 公开了处理和分选精子的方法。 精子分选或染色过程的一部分可以包括通过将精子样品的浓度调节到预定浓度并将样品的pH调节到预定值来标准化精子样品。 精子也可以在具有DNA选择性染料和淬灭染料的单一染色缓冲液中染色。