公开(公告)号：US09187764B2

公开(公告)日：2015-11-17

申请号：US14247145

申请日：2014-04-07

申请人： Institute of Hydrobiology, Chinese Academy of Sciences

发明人： Wei Hu ,  Yunsheng Zhang ,  Jun Dai ,  Zuoyan Zhu

IPC分类号： C12N15/85 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2217/15 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C12N2830/002

摘要： The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.

摘要翻译： 本发明公开了一种鱼繁殖控制方法，该方法包括：构建重组基因CMV-eGFP-SV40-CMV-Ga14-SV40，建立GAL4转基因斑马鱼系; B，构建重组基因CMV-RFP-SV40-UAS反义dnd，建立uas-antensednd转基因斑马鱼系; 和C，通过以两条线的纯合转基因鱼作为亲本杂交，并且杂交代鱼不育。 本发明的方法采取两条可施肥的杂交品系，但是杂交代为不育的策略，建立了一种具有控制鱼类生育能力的普遍意义的新方法; 该方法解决了鱼类繁殖不育与繁殖作业研究中人物遗传性的矛盾，可有效应用于新种鱼种群和群体控制的培育。