公开(公告)号：US20240358007A1

公开(公告)日：2024-10-31

申请号：US18141460

申请日：2023-04-30

Applicant: Vincenzo Michael Biella

Inventor： Vincenzo Michael Biella

IPC: A01K67/027 ,  C12N15/113 ,  C12N15/90

CPC classification number: A01K67/0276 ,  C12N15/113 ,  C12N15/907 ,  A01K2217/058 ,  A01K2217/075 ,  A01K2227/101 ,  C12N2310/14

Abstract: A bovine animal or offspring thereof or a bovine cell or gene comprising a modified chromosomal sequence in at least one allele of a gene encoding an Alpha-actinin-3 protein, wherein said modified chromosomal sequence partially or completely impairs the ability of said animal/cell/gene to encode said protein. The bovine retains all previous functionality, but is distinguished by a reduction in the number and cross-sectional area of Type II muscle fibers, and an increase in the proportion of Type I muscle fibers, resulting in improved feedlot efficiency, improved meat quality, and reduced emissions output.

公开(公告)号：US20180355352A1

公开(公告)日：2018-12-13

申请号：US15660556

申请日：2017-07-26

Applicant: Cancer Research Technology Limited

Inventor： Magdalena Zernicka-Goetz ,  Florence Wianny ,  Martin John Evans ,  David Moore Glover

IPC: C12N15/113 ,  C12N15/63 ,  C12N15/85 ,  C12N15/11 ,  A01K67/027 ,  A61K38/00

CPC classification number: C12N15/113 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2217/058 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C12N15/111 ,  C12N15/1137 ,  C12N15/1138 ,  C12N15/63 ,  C12N15/8509 ,  C12N2015/8527 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2330/50 ,  C12Y207/11001 ,  C12Y302/01031

Abstract: The present invention relates to the specific inhibition of gene expression in mammals by bringing the target gene into contact with double stranded RNA (dsRNA).

公开(公告)号：US20180334675A1

公开(公告)日：2018-11-22

申请号：US15904548

申请日：2018-02-26

Applicant: Duong Thanh Hoang ,  Kim Phuc Hoang

Inventor： Duong Thanh Hoang ,  Kim Phuc Hoang

IPC: C12N15/113 ,  A01K67/033

CPC classification number: C12N15/113 ,  A01K67/0333 ,  A01K67/0339 ,  A01K2217/058 ,  A01K2227/706 ,  A01K2267/02 ,  C12N2310/14 ,  C12N2330/51

Abstract: This invention entails a method to make a Tra-2 RNAi kernel sequence, and uses for this kernel sequence. The method includes the steps of amplifying an RNA recognition motive (RRM) DNA sequence from a Culcinae mosquito species, to obtain a first RRM DNA sequence of 240 base pairs; then reversely connecting the first RRM DNA sequence with a second RRM DNA sequence via an intron or a linker DNA sequence, in such a way that the second RRM DNA sequence forms an inverted sequence to the first RRM DNA sequence. Transcription of the two DNA sequences produces single strands of mRNA that can bind together to form a double strand hairpin mRNA structure. Other steps include inserting a tetracycline repressible transactivator, and an insect spermatogenesis promoter.

公开(公告)号：US20170165377A1

公开(公告)日：2017-06-15

申请号：US15423702

申请日：2017-02-03

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Phillip D. Zamore

IPC: A61K48/00 ,  A61K38/00 ,  C12N15/113 ,  C12N15/86 ,  C12N7/00

CPC classification number: A61K48/0008 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0331 ,  A61K9/0019 ,  A61K38/00 ,  A61K48/0058 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/8509 ,  C12N15/86 ,  C12N2015/8527 ,  C12N2310/13 ,  C12N2310/141 ,  C12N2320/32 ,  C12N2330/51 ,  C12N2710/10342 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2820/002 ,  C12N2840/007 ,  C12N2840/102

Abstract: The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.

公开(公告)号：US09596835B2

公开(公告)日：2017-03-21

申请号：US14940574

申请日：2015-11-13

Applicant: University of Massachusetts

Inventor： Guangping Gao ,  Phillip D. Zamore

IPC: C12N15/86 ,  A01K67/027 ,  C12N15/11 ,  C12N15/85

CPC classification number: A61K48/0008 ,  A01K67/0275 ,  A01K2207/05 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0331 ,  A61K9/0019 ,  A61K38/00 ,  A61K48/0058 ,  C12N7/00 ,  C12N15/111 ,  C12N15/113 ,  C12N15/8509 ,  C12N15/86 ,  C12N2015/8527 ,  C12N2310/13 ,  C12N2310/141 ,  C12N2320/32 ,  C12N2330/51 ,  C12N2710/10342 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2820/002 ,  C12N2840/007 ,  C12N2840/102

Abstract: The invention in some aspects relates to isolated nucleic acids, compositions, and kits useful for identifying adeno-associated viruses in cells. In some aspects, the invention provides kits and methods for producing somatic transgenic animal models using recombinant AAV (rAAV) to an animal having at least one transgene that expresses a small interfering nucleic acid or at least one binding site for a miRNA.

公开(公告)号：US09237740B2

公开(公告)日：2016-01-19

申请号：US13941014

申请日：2013-07-12

Applicant: The Jackson Laboratory

Inventor： Michael V. Wiles ,  Robert Taft

IPC: C12N15/00 ,  A01K67/02 ,  A01K67/027 ,  C12N15/877 ,  C12N15/873

CPC classification number: A01K67/02 ,  A01K67/0271 ,  A01K67/0275 ,  A01K2207/12 ,  A01K2217/058 ,  A01K2217/15 ,  A01K2217/203 ,  A01K2217/206 ,  A01K2217/30 ,  A01K2227/10 ,  A01K2267/02 ,  C12N15/873 ,  C12N15/877 ,  C12N15/8775

Abstract: Methods and compositions for producing selected non-human mammalian germ cells and gametes and for making non-human animals using the produced germ cells and gametes are provided by the present invention. Methods of generating a non-human embryo and/or animal derived from donor stem cells, methods of generating chimeric non-human animals having substantially all gametes and/or germ cells derived from the donor stem cells, methods of producing a non-human host embryo lacking functional endogenous germ cells and non-human host embryos incapable of developing endogenous gametes of the present invention are described herein.

Abstract translation: 通过本发明提供用于产生选择的非人哺乳动物生殖细胞和配子以及使用所产生的生殖细胞和配子制备非人动物的方法和组合物。 产生源自供体干细胞的非人胚胎和/或动物的方法，产生具有基本上所有来自供体干细胞的配子和/或生殖细胞的嵌合非人动物的方法，生产非人宿主的方法 本文描述了缺乏功能性内源性生殖细胞的胚胎和不能发育本发明的内源性配子的非人宿主胚胎。

公开(公告)号：US09228015B2

公开(公告)日：2016-01-05

申请号：US14146345

申请日：2014-01-02

Applicant: Biogen Idec MA Inc.

Inventor： Daniel H. S. Lee ,  Dingyi Wen ,  R. Blake Pepinsky ,  Jane K. Relton ,  Xinzhong Wang ,  Alexey Lugovskoy ,  Werner Meier ,  Ellen A. Garber ,  Laura Silvian ,  Paul H. Weinreb

IPC: C07K16/28 ,  A01K67/027 ,  C07K14/705 ,  C12N15/113 ,  C12N15/85 ,  A61K38/17 ,  A61K39/395 ,  A61K48/00 ,  A61K39/00

CPC classification number: C07K14/705 ,  A01K67/027 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/0356 ,  A61K38/179 ,  A61K39/3955 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/2863 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2319/30 ,  C07K2319/32 ,  C07K2319/33 ,  C07K2319/74 ,  C12N15/1136 ,  C12N15/1138 ,  C12N15/8509 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

Abstract: Disclosed are immunogenic Nogo receptor-1 polypeptides, Nogo receptor-1 antibodies, antigen-binding fragments thereof, soluble Nogo receptors and fusion proteins thereof and nucleic acids encoding the same. Also disclosed are Nogo receptor antagonist polynucleotides. Also disclosed are compositions comprising, and methods for making and using, such Nogo receptor antibodies, antigen-binding fragments thereof, soluble Nogo receptors and fusion proteins thereof, nucleic acids encoding the same and antagonist polynucleotides.

Abstract translation: 公开了免疫原性Nogo受体-1多肽，Nogo受体-1抗体，其抗原结合片段，可溶性Nogo受体及其融合蛋白和编码其的核酸。 还公开了Nogo受体拮抗剂多核苷酸。 还公开了包含这样的Nogo受体抗体，其抗原结合片段，可溶性Nogo受体及其融合蛋白，编码相同的核苷酸和拮抗剂多核苷酸的制备和使用方法。

公开(公告)号：US09187764B2

公开(公告)日：2015-11-17

申请号：US14247145

申请日：2014-04-07

Applicant: Institute of Hydrobiology, Chinese Academy of Sciences

Inventor： Wei Hu ,  Yunsheng Zhang ,  Jun Dai ,  Zuoyan Zhu

IPC: C12N15/85 ,  A01K67/027

CPC classification number: C12N15/8509 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2217/15 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C12N2830/002

Abstract: The invention discloses a method for controlling fish reproduction, the method comprises the following steps of: A, constructing a recombinant gene CMV-eGFP-SV40-CMV-Ga14-SV40 and establishing a GAL4 transgenic zebrafish line; B, constructing a recombinant gene CMV-RFP-SV40-UAS-antisense dnd and establishing a uas-antisensednd transgenic zebrafish line; and C, hybridizing by taking homozygous transgenic fish of the two lines as parents, and the hybrid generation fish was sterility. The method of the present invention takes a strategy that two fertilizable lines hybridized but hybrid generation is sterility, establishing a novel method with a universal meaning for controlling fish fertility; The method solves the contradiction of the reproductively sterility of fish and the hereditability of characters in a reproduction operation research and can be effectively applied to cultivation of new fish specie and population control.

Abstract translation: 本发明公开了一种鱼繁殖控制方法，该方法包括：构建重组基因CMV-eGFP-SV40-CMV-Ga14-SV40，建立GAL4转基因斑马鱼系; B，构建重组基因CMV-RFP-SV40-UAS反义dnd，建立uas-antensednd转基因斑马鱼系; 和C，通过以两条线的纯合转基因鱼作为亲本杂交，并且杂交代鱼不育。 本发明的方法采取两条可施肥的杂交品系，但是杂交代为不育的策略，建立了一种具有控制鱼类生育能力的普遍意义的新方法; 该方法解决了鱼类繁殖不育与繁殖作业研究中人物遗传性的矛盾，可有效应用于新种鱼种群和群体控制的培育。

公开(公告)号：US20120230938A1

公开(公告)日：2012-09-13

申请号：US13368796

申请日：2012-02-08

Applicant: David B. ROZEMA ,  Darren H. WAKEFIELD ,  David L. LEWIS ,  Jason KLEIN ,  So WONG ,  Jon A. WOLFF ,  James E. HAGSTROM

Inventor： David B. ROZEMA ,  Darren H. WAKEFIELD ,  David L. LEWIS ,  Jason KLEIN ,  So WONG ,  Jon A. WOLFF ,  James E. HAGSTROM

IPC: A61K31/787 ,  C08G63/91

CPC classification number: C12N15/87 ,  A01K2217/058 ,  A01K2267/0362 ,  A61K47/58 ,  A61K48/0041 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12N2800/95 ,  C12N2810/10

Abstract: The present invention is directed to compounds, compositions, and methods useful for delivering polynucleotides or other cell-impermeable molecules to mammalian cells. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery vehicles. The use of multiple reversible or labile linkages connecting component parts provides for physiologically responsive activity modulation.

Abstract translation: 本发明涉及可用于将多核苷酸或其它细胞不可渗透分子递送至哺乳动物细胞的化合物，组合物和方法。 描述了将靶向，抗调理作用，抗聚集和转染活性结合到小的生物相容性体内递送载体中的多偶联体系。 使用连接组成部分的多个可逆或不稳定的连接提供了生理响应活性调节。

公开(公告)号：US08217161B2

公开(公告)日：2012-07-10

申请号：US12427791

申请日：2009-04-22

Applicant: William S. Baldwin

Inventor： William S. Baldwin

IPC: C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12N5/02 ,  C12N15/11

CPC classification number: C12N15/1137 ,  A01K67/0275 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/0077 ,  C12N2310/14

Abstract: The present invention relates to siRNAs that are targeted to RNAs encoding two or more enzymes of a subfamily of cytochrome P450 (CYP) enzymes, along with vectors, cells, and kits comprising the siRNAs. The invention further relates to methods of decreasing expression of two or more CYP subfamily genes in a non-human animal, animals in which expression of two or more CYP subfamily genes has been decreased, and methods of using such animals to study the function of cytochrome P450 enzymes.

Abstract translation: 本发明涉及靶向编码细胞色素P450（CYP）酶亚家族的两种或多种酶的RNA以及载体，细胞和包含siRNA的试剂盒的siRNA。 本发明还涉及减少非人动物中两种或更多种CYP亚家族基因表达的方法，其中两种或更多种CYP亚族基因的表达降低的动物以及使用这些动物研究细胞色素的功能的方法 P450酶。