公开(公告)号：US20130019345A1

公开(公告)日：2013-01-17

申请号：US13634728

申请日：2010-03-17

申请人： Izuru Ohki ,  Ken-ichiro Taoka ,  Hiroyuki Tsuji ,  Chojiro Kojima ,  Ko Shimamoto

发明人： Izuru Ohki ,  Ken-ichiro Taoka ,  Hiroyuki Tsuji ,  Chojiro Kojima ,  Ko Shimamoto

IPC分类号： C07K14/415 ,  A01H5/00 ,  C12N15/63 ,  C12N15/29 ,  G06F19/16 ,  C07K1/107 ,  G01N21/64 ,  G01N24/08 ,  C12Q1/68 ,  C12N15/82 ,  G01N33/53

CPC分类号： A01H5/02 ,  C07K14/415 ,  C12N15/827 ,  C30B7/00 ,  C30B29/58 ,  G01N2500/00

摘要： Provided is a crystal of a florigen activation complex, including a florigen, a 14-3-3 protein, and a bZIP transcription factor bound to each other. In addition, flowering of a plant is regulated by controlling mechanisms of interactions among those proteins utilizing the crystal. These are achieved as follows. With attention focused on the fact that Hd3a is bound to FD1 via GF14c to form a florigen activation complex, a crystal of the florigen activation complex is produced, conformational information is obtained through the use of the crystal of the florigen activation complex, and the flowering of a plant is regulated by controlling mechanisms of interactions among the florigen and the like utilizing such conformational information.

摘要翻译： 提供了一种佛光激活复合物的晶体，其包括与其相互结合的花粉激素，14-3-3蛋白质和bZIP转录因子。 此外，通过控制利用晶体的那些蛋白质之间的相互作用机制来调节植物的开花。 这些实现如下。 注意到Hd3a通过GF14c与FD1结合以形成佛光激活复合物，产生了佛光激活复合物的晶体，通过使用佛光激活复合物的晶体获得构象信息，开花 通过利用这样的构象信息控制花椒等之间的相互作用机制来调节植物。

公开(公告)号：US5436391A

公开(公告)日：1995-07-25

申请号：US982712

申请日：1992-11-27

申请人： Hideya Fujimoto ,  Kimiko Ito ,  Mikihiro Yamamoto ,  Ko Shimamoto

发明人： Hideya Fujimoto ,  Kimiko Ito ,  Mikihiro Yamamoto ,  Ko Shimamoto

IPC分类号： A01H5/10 ,  C07K14/325 ,  A01H4/00 ,  C12N15/32

CPC分类号： C07K14/325 ,  A01H5/10

摘要： A synthetic gene encoding an insecticidal protein, the gene is provided having a base sequence which has been modified to bring the codon usage in conformity with the genes of gramineous plants particularly rice plants. A preferred gene sequence is shown in SEQ ID NO: 1. An expression vector containing said structural gene is provided. A method for producing recombinant gramineous plants is provided, which comprises transforming protoplasts derived from gramineous plants with the vector, incubating the protoplasts in a liquid medium in the presence of cultured cells of the plants to form colonies and regenerating plants from said colonies. Recombinant gramineous plants having resistance against insects are also provided.

摘要翻译： 编码杀虫蛋白的合成基因，提供了具有经修饰以使密码子使用与禾本科植物特别是水稻植物基因一致的碱基序列的基因。 优选的基因序列示于SEQ ID NO：1中。提供含有所述结构基因的表达载体。 提供了一种生产重组禾本科植物的方法，其包括用载体转化源自禾本科植物的原生质体，在培养的植物细胞存在下在液体培养基中孵育原生质体以形成菌落并从所述菌落再生植物。 还提供了具有抗昆虫的重组禾本科植物。

公开(公告)号：US5250433A

公开(公告)日：1993-10-05

申请号：US770766

申请日：1991-10-05

申请人： Ko Shimamoto ,  Junko Watanabe ,  Rie Terada ,  Yasuyuki Hayashi

发明人： Ko Shimamoto ,  Junko Watanabe ,  Rie Terada ,  Yasuyuki Hayashi

IPC分类号： A01H1/02 ,  A01H4/00 ,  C12N5/14 ,  C12N5/26

CPC分类号： A01H1/02 ,  A01H4/00 ,  C12N5/14

摘要： According to the present invention, there is provided a gramineous hybrid plant produced from a rice-derived protoplast and another gramineous plant-derived protoplast. Also provided by the invention is an efficient process for preparing such a gramineous hybrid plant wherein a protoplast is prepared from a callus or suspension cell of a rice plant (Oryza sativa), another protoplast is separately prepared from a callus or suspension cell of another gramineous plant, these protoplasts are subjected to cell fusion by electric treatment, the thus fused cells are cultivated in a culture medium containing cultured rice cells as nurse cells, and the thus formed colonies are then cultivated in a culture medium containing a plant hormone such as 2,4-dichlorophenoxyacetic acid.

摘要翻译： 根据本发明，提供了由米源原生质体和另一种禾本科植物来源的原生质体生产的禾本科杂交植物。 本发明还提供了一种制备这种禾本科杂交植物的有效方法，其中原生质体由水稻植物（水稻）的愈伤组织或悬浮细胞制备，另一种原生质体由另一种禾本科植物的愈伤组织或悬浮细胞分别制备 植物，通过电处理使这些原生质体进行细胞融合，将这样融合的细胞在含有培养的水稻细胞作为护士细胞的培养基中培养，然后将如此形成的菌落在含有植物激素如2的培养基中培养 ，4-二氯苯氧基乙酸。