公开(公告)号：US5367058A

公开(公告)日：1994-11-22

申请号：US111699

申请日：1993-08-25

申请人： J. Bruce Pitner ,  Linn, Jr. C. Preston ,  Patrick D. Mize

发明人： J. Bruce Pitner ,  Linn, Jr. C. Preston ,  Patrick D. Mize

IPC分类号： A61K49/16 ,  C07K15/28

CPC分类号： A61K49/16

摘要： The present invention relates to modified antibodies and fragments which display an increased affinity for antigen as compared to the affinity of unmodified antibody for antigen. The most preferred modification is the addition of a thiol group on the surface of the antibody such that a covalent bond forms between the antibody and antigen when they bind to one another.

摘要翻译： 本发明涉及与未修饰的抗体对抗原的亲和力相比显示增加的抗原亲和力的修饰的抗体和片段。 最优选的修饰是在抗体表面上添加硫醇基团，使得当它们彼此结合时，抗体和抗原之间形成共价键。

公开(公告)号：US20150018641A1

公开(公告)日：2015-01-15

申请号：US13932569

申请日：2013-07-01

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： A61B5/145 ,  A61B5/1459

CPC分类号： A61B5/14532 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4839 ,  A61B5/6848 ,  A61B2560/0223 ,  G01N21/274 ,  G01N21/7703 ,  G01N33/542 ,  G01N33/54373 ,  G01N2021/7786 ,  G01N2201/1211

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 感测元件连接到光学导管的远端，并且包含适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，光学导管和感测元件可以容纳在插管斜面内。

公开(公告)号：US07496392B2

公开(公告)日：2009-02-24

申请号：US10721797

申请日：2003-11-26

申请人： Javier Alarcón ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcón ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： A61B5/145

CPC分类号： G01N21/7703 ,  G01N33/542 ,  G01N33/54373

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is described. An optical conduit, preferably an optical fiber, has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and may include at least one binding protein adapted to bind with at least one target analyte. The sensing element further may also include at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

摘要翻译： 描述了用于感测体内或体外分析物浓度，特别是葡萄糖浓度的装置。 光导管，优选光纤，在光导管的近端具有光学系统。 感测元件连接到光学导管的远端，并且可以包括适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还可以包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，感测元件包括具有通过分析物浓度的变化基本上不变的发光特性的参考组。

公开(公告)号：US20110184259A1

公开(公告)日：2011-07-28

申请号：US12861202

申请日：2010-08-23

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. DeNuzzio ,  Christopher C. Herdman ,  Ross W. Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman ,  Steven Keith

IPC分类号： A61B5/1455

CPC分类号： A61B5/14532 ,  A61B5/1455 ,  A61B5/1459 ,  A61B5/1495 ,  A61B5/4839 ,  A61B5/6848 ,  A61B2560/0223 ,  G01N21/274 ,  G01N21/7703 ,  G01N33/542 ,  G01N33/54373 ,  G01N2021/7786 ,  G01N2201/1211

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 感测元件连接到光学导管的远端，并且包含适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，光学导管和感测元件可以容纳在插管斜面内。

公开(公告)号：US20080198358A1

公开(公告)日：2008-08-21

申请号：US11840069

申请日：2007-08-16

申请人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

发明人： Javier Alarcon ,  Kristin Weidemaier ,  Terry J. Amiss ,  John D. Denuzzio ,  Christopher C. Herdman ,  Ross Wayne Jacobson ,  J. Bruce Pitner ,  Douglas B. Sherman

IPC分类号： G03B27/72

CPC分类号： G01N21/7703 ,  G01N33/542 ,  G01N33/54373

摘要： A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. An optical conduit, preferably an optical fiber has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

摘要翻译： 公开了用于感测体内或体外的分析物浓度，特别是葡萄糖浓度的装置。 光导管，优选地，光导纤维在光导管的近端具有光学系统。 感测元件连接到光学导管的远端，并且包括适于与至少一种靶分析物结合的至少一种结合蛋白。 感测元件还包括至少一个报告基团，其随着分析物浓度的变化而发生发光变化。 可选地，感测元件包括具有通过分析物浓度的变化基本上不变的发光特性的参考组。

公开(公告)号：US06576430B1

公开(公告)日：2003-06-10

申请号：US09716566

申请日：2000-11-20

申请人： Helen V. Hsieh ,  J. Bruce Pitner ,  Jason E. Gestwicki

发明人： Helen V. Hsieh ,  J. Bruce Pitner ,  Jason E. Gestwicki

IPC分类号： G01N3353

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S435/964 ,  Y10S436/805 ,  Y10T436/24

摘要： Methods for detection and analysis of allosteric receptor/ligand binding by changes in surface refractive index are provided. When analyzed by surface plasmon resonance, binding of such allosteric binding agents to their ligands may result in a negative deviation in the optical response (i.e., a decrease in resonance angle) or in an increase in the optical response (i.e., an increase in resonance angle) depending on the binding properties of the selected allosteric receptor. Other methods for analysis of surface refractive index are also useful in the invention. The methods of the invention are particularly useful for small ligands which would not be expected to produce detectable changes in refractive index because they do not add significant mass upon binding.

摘要翻译： 提供了通过表面折射率变化检测和分析变构受体/配体结合的方法。 当通过表面等离子体共振分析时，这种变构结合剂与其配体的结合可能导致光学响应的​​负偏差（即，共振角的减小）或光响应的增加（即共振增加 角度）取决于所选择的变构受体的结合特性。 用于分析表面折射率的其它方法在本发明中也是有用的。 本发明的方法对于小的配体特别有用，因为它们在结合时不会增加显着的质量，所以不希望产生可检测的折射率变化。

公开(公告)号：US06395506B1

公开(公告)日：2002-05-28

申请号：US09342720

申请日：1999-06-29

申请人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

发明人： J. Bruce Pitner ,  John Jacob Hemperly ,  Richard D. Guarino ,  Magdalena Wodnicka ,  David T. Stitt ,  Gregory J. Burrell ,  Timothy G. Foley, Jr. ,  Patrick Shawn Beaty

IPC分类号： C12Q118

CPC分类号： C12Q1/18 ,  C12Q1/04 ,  C12Q2304/44 ,  G06F19/00 ,  Y02A90/26

摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。

公开(公告)号：US5837695A

公开(公告)日：1998-11-17

申请号：US701340

申请日：1996-08-22

申请人： J. Bruce Pitner

发明人： J. Bruce Pitner

IPC分类号： A61K31/70 ,  C07H15/24

CPC分类号： C07H13/10

摘要： The present invention relates to a unique fluorescently labeled Chlamydial antigen. This fluorescently labeled Chlamydial antigen is useful in immunoassay-type formatted systems for the detection of antibodies to Chlamydia or unlabeled Chlamydia antigens.

摘要翻译： 本发明涉及独特的荧光标记衣原体抗原。 这种荧光标记的衣原体抗原可用于免疫测定型格式化系统，用于检测衣原体或未标记衣原体抗原的抗体。

公开(公告)号：US5380880A

公开(公告)日：1995-01-10

申请号：US163099

申请日：1993-12-06

申请人： J. Bruce Pitner ,  Randal A. Hoke

发明人： J. Bruce Pitner ,  Randal A. Hoke

IPC分类号： C09B11/28 ,  C07D311/82 ,  C09B11/24 ,  C09K11/06 ,  G01N21/80 ,  G01N31/22 ,  C07D311/88

CPC分类号： C09B11/24 ,  C07D311/82 ,  G01N31/221

摘要： Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.

摘要翻译： 荧光pH指示剂化合物，随着pH值的降低，荧光强度呈现增加的趋势。 指示剂是罗丹明和磺酰罗丹明型染料的衍生物，并且特别可用于在酸性环境中测量pH，例如微生物产生的二氧化碳和某些酸性细胞内区室的pH。

公开(公告)号：US5302731A

公开(公告)日：1994-04-12

申请号：US912426

申请日：1992-07-13

申请人： J. Bruce Pitner ,  Randal A. Hoke

发明人： J. Bruce Pitner ,  Randal A. Hoke

IPC分类号： C09B11/28 ,  C07D311/82 ,  C09B11/24 ,  C09K11/06 ,  G01N21/80 ,  G01N31/22 ,  C07D311/90

CPC分类号： C09B11/24 ,  C07D311/82 ,  G01N31/221

摘要： Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.

摘要翻译： 荧光pH指示剂化合物，随着pH值的降低，荧光强度呈现增加的趋势。 指示剂是罗丹明和磺酰罗丹明型染料的衍生物，并且特别可用于在酸性环境中测量pH，例如微生物产生的二氧化碳和某些酸性细胞内区室的pH。