公开(公告)号：US10128078B2

公开(公告)日：2018-11-13

申请号：US15114978

申请日：2015-01-28

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Satoshi Hirakawa ,  Hideaki Kawasaki ,  Masatsugu Shimomura ,  Daisuke Ishii ,  Isao Ohta ,  Yoshinori Muranaka

IPC: H01J37/20 ,  G01N33/483 ,  G01N33/50 ,  H01J37/22

Abstract: Provided are a protective agent for electron microscopic observation in a vacuum which can protect a biological sample in a water-containing state, such as a mammal, a plant tissue or a cultured cell, and a single cell in the living state without deforming it even in a vacuum, a kit using the same, methods for observation, diagnosis, evaluation, and quantification of a sample by an electron microscope, and a sample stage to be used for the observation. The protective agent for electron microscopic observation of the present invention contains a a component to impart the survival environment, a saccharide, and an electrolyte.

公开(公告)号：US10046283B2

公开(公告)日：2018-08-14

申请号：US15599841

申请日：2017-05-19

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Masatsugu Shimomura ,  Daisuke Ishii ,  Isao Ohta ,  Yoshinori Muranaka

IPC: B01D69/02 ,  B01D71/38 ,  C09D4/00 ,  C09D4/06 ,  B01D67/00 ,  B01D69/12

Abstract: Provided is a technique for fabricating a novel thin membrane with a starting material such as a biocompatible compound that is not easily processible into a membrane, particularly a technique for fabricating a novel thin membrane of a composition that is gradually polymerized from a membrane surface into the membrane in the cross sectional direction of the membrane, and having, for example, different structures on the front and back of the membrane. The technique includes the steps of preparing a solution of a starting material compound; forming a thin membrane of the solution on a base material surface; and forming the organic polymer thin membrane through a polymerization reaction caused by irradiating the exposed surface of the thin membrane with a plasma or an electron beam.

公开(公告)号：US09687793B2

公开(公告)日：2017-06-27

申请号：US14426508

申请日：2013-09-06

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Masatsugu Shimomura ,  Daisuke Ishii ,  Isao Ohta ,  Yoshinori Muranaka

IPC: B01D69/12 ,  B01D71/38 ,  C09D4/00 ,  C09D4/06 ,  B01D67/00 ,  B01D69/02

CPC classification number: B01D71/38 ,  B01D67/009 ,  B01D69/02 ,  B01D69/127 ,  B01D2323/30 ,  B01D2325/36 ,  C09D4/00 ,  C09D4/06

Abstract: Provided is a technique for fabricating a novel thin membrane with a starting material such as a biocompatible compound that is not easily processible into a membrane, particularly a technique for fabricating a novel thin membrane of a composition that is gradually polymerized from a membrane surface into the membrane in the cross sectional direction of the membrane, and having, for example, different structures on the front and back of the membrane. The technique includes the steps of preparing a solution of a starting material compound; forming a thin membrane of the solution on a base material surface; and forming the organic polymer thin membrane through a polymerization reaction caused by irradiating the exposed surface of the thin membrane with a plasma or an electron beam.

公开(公告)号：US20140227734A1

公开(公告)日：2014-08-14

申请号：US14343537

申请日：2012-09-07

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Yoshinori Muranaka ,  Isao Ohta ,  Masatsugu Shimomura ,  Daisuke Ishii

IPC: G01N1/44 ,  G01N23/225

CPC classification number: G01N1/44 ,  G01N1/2853 ,  G01N23/04 ,  G01N23/225 ,  G01N23/2252 ,  G01N2223/612 ,  H01J37/20 ,  H01J2237/2002 ,  H01J2237/2004

Abstract: Provided is an observation method by an electron microscope, in which a biological sample can be observed as it is alive and a situation that the biological sample is moving can be observed using an electron microscope, and a composition for evaporation suppression under vacuum, a scanning electron microscope, and a transmission electron microscope used in the method.The sample observation method by an electron microscope according to the invention includes applying a composition for evaporation suppression containing at least one kind selected from an amphiphilic compound, oils and fats, and an ionic liquid to the surface of a sample to form a thin film, and covering the sample with the thin film, and displaying an electron microscopic image of the sample, which is covered with the thin film and accommodated in a sample chamber under vacuum, on a display device.

Abstract translation: 提供一种电子显微镜的观察方法，其中可以观察生物样品的活性，并且可以使用电子显微镜观察生物样品移动的情况，以及用于真空下蒸发抑制的组合物，扫描 电子显微镜和用于该方法的透射电子显微镜。 通过根据本发明的电子显微镜的样品观察方法包括将包含选自两亲性化合物，油和脂肪中的至少一种的蒸发抑制组合物和离子液体施加到样品的表面以形成薄膜， 并用薄膜覆盖样品，并在显示装置上显示被薄膜覆盖并在真空下容纳在样品室中的样品的电子显微镜图像。

公开(公告)号：US20150224452A1

公开(公告)日：2015-08-13

申请号：US14426508

申请日：2013-09-06

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko Hariyama ,  Yasuharu Takaku ,  Hiroshi Suzuki ,  Masatsugu Shimomura ,  Daisuke Ishii ,  Isao Ohta ,  Yoshinori Muranaka

IPC: B01D71/38 ,  B01D69/12 ,  B01D69/02 ,  B01D67/00

CPC classification number: B01D71/38 ,  B01D67/009 ,  B01D69/02 ,  B01D69/127 ,  B01D2323/30 ,  B01D2325/36 ,  C09D4/00 ,  C09D4/06

Abstract: Provided is a technique for fabricating a novel thin membrane with a starting material such as a biocompatible compound that is not easily processible into a membrane, particularly a technique for fabricating a novel thin membrane of a composition that is gradually polymerized from a membrane surface into the membrane in the cross sectional direction of the membrane, and having, for example, different structures on the front and back of the membrane. The technique includes the steps of preparing a solution of a starting material compound; forming a thin membrane of the solution on a base material surface; and forming the organic polymer thin membrane through a polymerization reaction caused by irradiating the exposed surface of the thin membrane with a plasma or an electron beam.

Abstract translation: 本发明提供一种制备具有不容易加工成膜的生物相容性化合物等起始材料的新型薄膜的技术，特别是制造从膜表面逐渐聚合的新型薄膜的技术， 膜在膜的横截面方向上，并且在膜的前后具有例如不同的结构。 该技术包括制备原料化合物溶液的步骤; 在基材表面上形成薄膜溶液; 以及通过用等离子体或电子束照射薄膜的暴露表面而引起的聚合反应形成有机聚合物薄膜。