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公开(公告)号:US20100317540A1
公开(公告)日:2010-12-16
申请号:US12772829
申请日:2010-05-03
申请人: JOHN MCCAFFERTY , ANTHONY RICHARD POPE , KEVIN STUART JOHNSON , HENDRICUS RENERUS JACOBUS MATTHEUS HOOGENBOOM , ANDREW DAVID GRIFFITHS , RONALD HENRY JACKSON , KASPER PHILIPP HOLLIGER , JAMES DAVID MARKS , TIMOTHY PIERS CLACKSON , DAVID JOHN CHISWELL , GREGORY PAUL WINTER , TIMOTHY PETER BONNERT
发明人: JOHN MCCAFFERTY , ANTHONY RICHARD POPE , KEVIN STUART JOHNSON , HENDRICUS RENERUS JACOBUS MATTHEUS HOOGENBOOM , ANDREW DAVID GRIFFITHS , RONALD HENRY JACKSON , KASPER PHILIPP HOLLIGER , JAMES DAVID MARKS , TIMOTHY PIERS CLACKSON , DAVID JOHN CHISWELL , GREGORY PAUL WINTER , TIMOTHY PETER BONNERT
CPC分类号: C07K16/005 , B01J2219/00718 , C07K16/00 , C07K16/18 , C07K16/26 , C07K16/34 , C07K16/40 , C07K16/44 , C07K16/462 , C07K16/468 , C07K19/00 , C07K2317/21 , C07K2317/24 , C07K2317/34 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/92 , C07K2319/00 , C07K2319/02 , C07K2319/41 , C07K2319/735 , C12N15/1037 , C12N15/62 , C12N15/73 , C40B40/02 , G01N33/53 , G01N33/6857 , Y10S530/867
摘要: A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.