公开(公告)号：US20120058907A1

公开(公告)日：2012-03-08

申请号：US13200972

申请日：2011-10-05

申请人： Ton Logtenberg ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Ton Logtenberg ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： C40B30/04 ,  C12N15/63 ,  C12N15/81 ,  C12N15/85 ,  C12N15/80

CPC分类号： C12N15/1086 ,  C07K16/10 ,  C07K16/20 ,  C07K16/40 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2318/20 ,  C12N15/1082

摘要： Described are methods for producing libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.

摘要翻译： 描述了用于产生表达至少两个单独的多肽链结合蛋白的细胞文库的方法，其中结合蛋白具有不同的靶表位。 通过将编码多肽链的核酸序列整合到宿主细胞的基因组中并选择成功整合这些核酸的细胞来制备此类文库。 所选择的细胞优选进行克隆步骤。 产生结合蛋白的混合物，而不必单独产生混合物的每种组分。 还提供了其中基本上每个细胞编码至少两种具有不同靶表位的单个多肽链结合蛋白的细胞库，以及用于产生包含至少两种具有不同靶表位的单独多肽链结合蛋白的组合物的方法。

公开(公告)号：US08975382B2

公开(公告)日：2015-03-10

申请号：US12744991

申请日：2008-11-27

申请人： Hilde Adi Pierrette Revets ,  Carlo Boutton ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Hilde Adi Pierrette Revets ,  Carlo Boutton ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： C07K16/30 ,  C07K16/32 ,  C07K16/46 ,  C07K16/28

CPC分类号： C07K16/2863 ,  A61K39/3955 ,  A61K2039/505 ,  C07K16/3007 ,  C07K16/32 ,  C07K16/468 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/32 ,  C07K2317/52 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

摘要： The present invention relates to amino acid sequences and Nanobodies that are directed against Epidermal Growth Factor Receptor 2 (HER2), as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

摘要翻译： 本发明涉及针对表皮生长因子受体2（HER2）的氨基酸序列和纳米抗体，以及包含或基本上由一个或多个这样的氨基酸组成的化合物或构建体，特别是蛋白质和多肽 序列。

公开(公告)号：US08907065B2

公开(公告)日：2014-12-09

申请号：US12448260

申请日：2007-12-17

申请人： Guy Hermans ,  Peter Verheesen ,  Edward Dolk ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Renee de Bruin

发明人： Guy Hermans ,  Peter Verheesen ,  Edward Dolk ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Michael John Scott Saunders ,  Johannes Joseph Wilhelmus De Haard ,  Renee de Bruin

IPC分类号： C07K16/28 ,  A61K39/395

CPC分类号： C07K16/2818 ,  A61K47/643 ,  C07K14/76 ,  C07K16/2803 ,  C07K16/2827 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that block the interaction between (a target on) an antigen presenting cell (APC) and (a target on) a T-cell. More particularly, the present invention relates to amino acid sequences that are directed against (as defined herein) a target on an APC (also referred to herein as “APC target”) or a target on a T-cell (also referred to herein as “T-cell target”). The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences.

摘要翻译： 本发明涉及阻断抗原呈递细胞（APC）上的靶标（APC）和（T细胞上的靶标）之间的相互作用的氨基酸序列。 更具体地，本发明涉及针对（如本文所定义）APC上的靶（本文中也称为“APC靶”）或T细胞上的靶（本文也称为“靶”）的氨基酸序列 “T细胞靶”）。 本发明还涉及包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。

公开(公告)号：US20120177637A1

公开(公告)日：2012-07-12

申请号：US12931955

申请日：2011-02-14

申请人： Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Ton Logtenberg

发明人： Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Ton Logtenberg

IPC分类号： A61K39/42 ,  C40B40/02 ,  C12N5/10 ,  A61K39/395 ,  C12P19/34 ,  A61P31/14 ,  C12P21/00 ,  C40B30/04

CPC分类号： G01N33/56966 ,  C07K16/005 ,  C07K16/10 ,  C07K16/22 ,  C07K16/248 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/00

摘要： The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising paired variable regions, the at least two proteinaceous molecules having different binding specificities, comprising paired variable regions, at least two proteinaceous molecules having different binding specificities, comprising contacting at least three different variable regions under conditions allowing for pairing of variable regions and harvesting essentially all proteinaceous molecules having binding specificities resulting from the pairing.

摘要翻译： 本发明提供了被设计为真实组合的特异性结合蛋白（例如免疫球蛋白）的组合，该组合的基本上所有组分都是功能性的并且彼此相容。 本发明进一步提供一种制备包含至少两种不同蛋白质分子的组合物的方法，所述蛋白质分子包含配对可变区，所述至少两种具有不同结合特异性的蛋白质分子包含配对的可变区，至少两种具有不同结合特异性的蛋白质分子，包括接触 至少三个不同的可变区，在允许可变区配对和收获基本上所有具有由配对产生的结合特异性的蛋白质分子的条件下。

公开(公告)号：US08188223B2

公开(公告)日：2012-05-29

申请号：US11587749

申请日：2006-05-17

申请人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K16/46

CPC分类号： C07K16/241 ,  A61K2039/505 ,  C07K14/525 ,  C07K16/18 ,  C07K16/468 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that are capable of binding to serum albumin, which sequences do not significantly reduce or inhibit the binding of serum albumin to FcRn or significantly reduce the half-life of serum albumin. It further relates to proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides.

摘要翻译： 本发明涉及能够结合血清白蛋白的氨基酸序列，该序列不显着降低或抑制血清白蛋白与FcRn的结合或显着降低血清白蛋白的半衰期。 它还涉及包含或基本上由这样的氨基酸序列组成的蛋白质和多肽; 涉及编码这种氨基酸序列，蛋白质或多肽的核酸; 包括这样的氨基酸序列，蛋白质和多肽的组合物，特别是药物组合物; 以及这些氨基酸序列，蛋白质和多肽的使用。

公开(公告)号：US20120129710A1

公开(公告)日：2012-05-24

申请号：US13165300

申请日：2011-06-21

申请人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

发明人： John McCafferty ,  Anthony Richard Pope ,  Kevin Stuart Johnson ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Andrew David Griffiths ,  Ronald Henry Jackson ,  Kasper Philipp Holliger ,  James David Marks ,  Timothy Piers Clackson ,  David John Chiswell ,  Gregory Paul Winter ,  Timothy Peter Bonnert

IPC分类号： C40B30/04 ,  C12N7/01 ,  G01N33/53 ,  C12N15/63

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特异性结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的遗传多样性群体的DNA来鉴定，其中sbp成员以分泌重组遗传显示包（rgdp）的表面以功能形式显示 ），其含有编码sbp成员或其多肽组分的DNA，其中sbp成员或其多肽组分被表达为与rgdp的衣壳组分的融合物。 显示的sbps可以通过与补充sbp成员的亲和力来选择，并且从所选的rgdps中恢复的DNA用于表达所选择的sbp成员。 因此可以获得抗体sbp成员，其中不同的链表达，一个与衣壳组分融合，另一个与游离形式融合，用于与融合伴侣多肽结合。 噬菌粒可以用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以组合编码这种多聚体sbp成员的各个链的DNA文库，从而获得在sbp成员中比通过常规方法容易地获得的更大的遗传多样性。

公开(公告)号：US20100104568A1

公开(公告)日：2010-04-29

申请号：US12558032

申请日：2009-09-11

申请人： Els Anna Alice Beirnaert ,  Sigrid Cornelis ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Els Anna Alice Beirnaert ,  Sigrid Cornelis ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K14/00 ,  C07K16/00 ,  C07H21/04 ,  C12N5/16 ,  A61K38/16 ,  C12P21/06

CPC分类号： C07K16/2875 ,  A61K39/3955 ,  A61K47/60 ,  A61K47/6849 ,  A61K2039/505 ,  C07K14/765 ,  C07K16/24 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that are directed against RANK-L, as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The invention also relates to nucleic acids encoding such amino acid sequences and polypeptides; to methods for preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.

摘要翻译： 本发明涉及针对RANK-L的氨基酸序列，以及包含或基本上由一个或多个这样的氨基酸序列组成的化合物或构建体，特别是蛋白质和多肽。 本发明还涉及编码这种氨基酸序列和多肽的核酸; 涉及制备这种氨基酸序列和多肽的方法; 宿主表达或能够表达这种氨基酸序列或多肽的细胞; 包括这样的氨基酸序列，多肽，核酸和/或宿主细胞的组合物，特别是药物组合物; 以及这些氨基酸序列或多肽，核酸，宿主细胞和/或组合物的用途，特别是用于预防，治疗或诊断目的。

公开(公告)号：US20090074770A1

公开(公告)日：2009-03-19

申请号：US11974186

申请日：2007-10-11

申请人： Ignace Joseph Isabella Lasters ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Ignace Joseph Isabella Lasters ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K14/00 ,  C07K16/18 ,  C12N15/11 ,  A61P43/00 ,  C12N5/06 ,  C12P21/04 ,  A61K38/00

CPC分类号： C07K14/001 ,  A61K38/00 ,  C12N15/1037

摘要： The present invention relates to amino acid sequences that binding to serum proteins such as serum albumin; to compounds, proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides, is essentially independent in the pH range 5 to 8.

摘要翻译： 本发明涉及与血清蛋白如血清白蛋白结合的氨基酸序列; 包括或基本上由这样的氨基酸序列组成的化合物，蛋白质和多肽; 涉及编码这种氨基酸序列，蛋白质或多肽的核酸; 包括这样的氨基酸序列，蛋白质和多肽的组合物，特别是药物组合物; 并且使用这些氨基酸序列，蛋白质和多肽，在5至8的pH范围内基本上是独立的。

公开(公告)号：US20080267949A1

公开(公告)日：2008-10-30

申请号：US11999497

申请日：2007-12-05

申请人： Hilde Adi Pierrette Revets ,  Joost Alexander Kolkman ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

发明人： Hilde Adi Pierrette Revets ,  Joost Alexander Kolkman ,  Hendricus Renerus Jacobus Mattheus Hoogenboom

IPC分类号： A61K39/395 ,  C07K14/435 ,  C07H21/00 ,  C12N5/10 ,  A61K38/16 ,  C40B30/04 ,  A61P43/00

CPC分类号： C07K16/18 ,  C07K16/32 ,  C07K2317/22 ,  C07K2317/31 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2319/31 ,  G01N33/5047 ,  G01N33/6857

摘要： The present invention relates to amino acid sequences that are capable of binding to serum proteins; to compounds, proteins, polypeptides, fusion proteins or constructs comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs; and to uses of such amino acid sequences, compounds, proteins, polypeptides, fusion proteins or constructs.

摘要翻译： 本发明涉及能够结合血清蛋白质的氨基酸序列; 化合物，蛋白质，多肽，融合蛋白或包含或基本上由此类氨基酸序列组成的构建体; 编码这种氨基酸序列的核酸，化合物，蛋白质，多肽，融合蛋白或构建体; 包括这样的氨基酸序列，化合物，蛋白质，多肽，融合蛋白或构建体的组合物，特别是药物组合物; 以及这些氨基酸序列，化合物，蛋白质，多肽，融合蛋白或构建体的使用。

公开(公告)号：US20070269422A1

公开(公告)日：2007-11-22

申请号：US11517816

申请日：2006-09-08

申请人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Heidi Maria Florence Jonckheere ,  Torsten Dreier

发明人： Els Beirnaert ,  Hilde Adi Pierrette Revets ,  Hendricus Renerus Jacobus Mattheus Hoogenboom ,  Heidi Maria Florence Jonckheere ,  Torsten Dreier

IPC分类号： A61K39/395 ,  A61K31/7052 ,  A61K48/00 ,  C07H21/04 ,  C07K14/00 ,  C07K16/18 ,  C12N5/06 ,  C12P1/04

CPC分类号： C07K16/18 ,  C07K16/241 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2319/31

摘要： The present invention relates to amino acid sequences that are capable of binding to serum albumin; to compounds, proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides. Particularly, the amino acid sequences and compounds of the present invention bind to or otherwise associate with serum albumin in such a way that, when the amino acid sequence or compound is bound to or otherwise associated with a serum albumin molecule in a primate, it exhibits a serum half-life of at least 50% of the natural half-life of serum albumin in said primate.

摘要翻译： 本发明涉及能够结合血清白蛋白的氨基酸序列; 包括或基本上由这样的氨基酸序列组成的化合物，蛋白质和多肽; 涉及编码这种氨基酸序列，蛋白质或多肽的核酸; 包括这样的氨基酸序列，蛋白质和多肽的组合物，特别是药物组合物; 以及这些氨基酸序列，蛋白质和多肽的使用。 特别地，本发明的氨基酸序列和化合物与血清白蛋白结合或以其它方式结合，使得当氨基酸序列或化合物与灵长类动物中的血清白蛋白分子结合或以其他方式结合时，其显示出 所述灵长类动物中血清白蛋白的天然半衰期至少为50％的血清半衰期。