公开(公告)号：US20220017896A1

公开(公告)日：2022-01-20

申请号：US17296629

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N15/11 ,  C12N9/22

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Defluviimonas sp. 20V17, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and specific conditions.

公开(公告)号：US20220002692A1

公开(公告)日：2022-01-06

申请号：US17296597

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Clostridium celluloliticum, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and in wider temperature ranges. Further, provided is more facile editing of the genome of the biotechnologically significant bacterium Clostridium celluloliticum.