公开(公告)号：US20220017896A1

公开(公告)日：2022-01-20

申请号：US17296629

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N15/11 ,  C12N9/22

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Defluviimonas sp. 20V17, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and specific conditions.

公开(公告)号：US20220064612A1

公开(公告)日：2022-03-03

申请号：US17276016

申请日：2019-09-13

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Dmitriy Aleksandrovich MADERA ,  Aleksandr Vladimirovich KARABELSKII ,  Roman Alekseevich IVANOV ,  Dmitry Valentinovich MOROZOV ,  Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Dmitrii Aleksandrovich SUTORMIN ,  Georgii Evgenevich POBEGALOV ,  Aleksandra Andreevna VASILEVA ,  Polina Anatolevna SELKOVA ,  Anatolii Nikolaevich ARSENIEV ,  Tatyana Igorevna ZYUBKO ,  Iana Vitalevna FEDOROVA

IPC: C12N9/22 ,  C12N15/79

Abstract: The present invention relates to the field of biotechnology, molecular biology and medicine, in particular to nuclease enzyme and use thereof. More specifically, the present invention relates to PaCas9 nuclease enzyme. The invention also relates to a nucleic acid encoding said nuclease, a genetic construct, an expression vector, a delivery vector, which comprise said nucleic acid, a liposome comprising said nuclease or nucleic acid encoding said nuclease, a method for producing a nuclease, methods for delivery, and a host cell comprising a nucleic acid encoding said nuclease.

公开(公告)号：US20220002692A1

公开(公告)日：2022-01-06

申请号：US17296597

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Clostridium celluloliticum, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and in wider temperature ranges. Further, provided is more facile editing of the genome of the biotechnologically significant bacterium Clostridium celluloliticum.