公开(公告)号：US4770781A

公开(公告)日：1988-09-13

申请号：US80182

申请日：1987-07-29

申请人： Jack A. Schmidt ,  Patricia M. Cameron ,  Guadalupe A. Limjuco

发明人： Jack A. Schmidt ,  Patricia M. Cameron ,  Guadalupe A. Limjuco

IPC分类号： B01D15/08 ,  B01J41/20 ,  C07K14/545

CPC分类号： B01J41/20 ,  B01D15/363 ,  C07K14/545 ,  B01D15/325 ,  B01D15/34

摘要： Human interleukin-1 (IL-1) charged species (pI 6.8, 6.0, 5.4 and 5.2) are separated and purified by a rapid and efficient two or three stage chromatographic process. Interleukin-1 species present in concentrated culture fluid from stimulated human mononuclear cells are separated and partially purified by anion exchange high performance liquid chromatography. The pI 5.4 and 5.2 species in addition are partially purified by HPLC gel filtration chromatography. Each of the individual IL-1 species are then finally purified by reverse phase high performance liquid chromatography. This process results in homogenously pure pI 6.8, pI 5.4 and pI 5.2 species while the pI 6.0 species is isolated in a substantially pure form.

摘要翻译： 通过快速和有效的两级或三级色谱法分离和纯化人白细胞介素-1（IL-1）带电物种（pI 6.8,6.0,5.4和5.2）。 通过阴离子交换高效液相色谱分离来自刺激的人单核细胞的浓缩培养液中存在的白细胞介素-1物质。 另外通过HPLC凝胶过滤色谱法部分纯化pI 5.4和5.2物质。 然后通过反相高效液相色谱将每种单独的IL-1物质进行纯化。 该过程产生均匀纯的pI 6.8，pI 5.4和pI 5.2物种，而pI 6.0物质以基本上纯的形式分离。

公开(公告)号：US4285931A

公开(公告)日：1981-08-25

申请号：US122092

申请日：1980-02-15

申请人： Guadalupe A. Limjuco ,  Yashwant D. Karkhanis ,  Dennis J. Carlo

发明人： Guadalupe A. Limjuco ,  Yashwant D. Karkhanis ,  Dennis J. Carlo

IPC分类号： A61K39/02 ,  A61K39/108 ,  A61K37/02

CPC分类号： A61K39/0258 ,  Y10S530/825

摘要： A vaccine against E. coli caused diarrhea is prepared from an isolate of the homogeneous heat-labile enterotoxin of human strain MB3353 of Escherichia coli, ATCC 31361, or of the porcine strain MB3708, ATCC 31362. The toxin, which is a protein, is a low molecular weight species of 10,000-13,000.

摘要翻译： 从大肠杆菌，ATCC 31361或人类MB3708，ATCC 31362的人源MB3353的均质热不稳定肠毒素的分离物制备针对大肠杆菌引起的腹泻的疫苗。作为蛋白质的毒素是 10,000-13,000的低分子量物种。

公开(公告)号：US4220584A

公开(公告)日：1980-09-02

申请号：US873181

申请日：1978-01-30

申请人： Guadalupe A. Limjuco ,  Yashwant D. Karkhanis ,  Dennis J. Carlo

发明人： Guadalupe A. Limjuco ,  Yashwant D. Karkhanis ,  Dennis J. Carlo

IPC分类号： C12N1/20 ,  A61K39/02 ,  A61K39/108 ,  A61K37/02

CPC分类号： A61K39/0258 ,  Y10S530/806 ,  Y10S530/825

摘要： A vaccine against E. coli caused diarrhea is prepared from an isolate of the homogeneous heat-labile enterotoxin of human strain MB3353 of Escherichia coli, ATCC 31361, or of the porcine strain MB3708, ATCC 31362. The toxin, which is a protein, is a low molecular weight species of 10,000-13,000.

摘要翻译： 从大肠杆菌，ATCC 31361或人类MB3708，ATCC 31362的人源MB3353的均质热不稳定肠毒素的分离物制备针对大肠杆菌引起的腹泻的疫苗。作为蛋白质的毒素是 10,000-13,000的低分子量物种。

公开(公告)号：US3978209A

公开(公告)日：1976-08-31

申请号：US560767

申请日：1975-03-24

申请人： Guadalupe A. Limjuco ,  Dennis J. Carlo

发明人： Guadalupe A. Limjuco ,  Dennis J. Carlo

IPC分类号： A61K39/095 ,  A61K39/02

CPC分类号： A61K39/095

摘要： Endotoxin contaminant in meningococcus group A and group C polysaccharide is removed by means of a phosphate containing adsorbent.