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公开(公告)号：US07611852B2
公开(公告)日：2009-11-03
申请号：US10627245
申请日：2003-07-25
申请人： James A. Thomson , Timothy J. Kamp , Yue Ma , Jia-Qiang He
发明人： James A. Thomson , Timothy J. Kamp , Yue Ma , Jia-Qiang He
IPC分类号： G01N33/567 , C12N5/08 , C12N5/00 , C12N5/02
CPC分类号： G01N33/5008 , G01N33/5014 , G01N33/502 , G01N33/5061 , G01N33/5073 , G01N33/5088
摘要： Human embryonic stem cells form embryoid bodies in culture which contain differentiated human cells. Some of the human cells in embryoid bodies differentiate into cardiomyocytes. Here the biological and electrical characteristics of those cardiomyocytes are described with reference to the use of cardiomyocytes derived from human embryonic stem cells in drug screening protocols for mechanisms of cardiac toxicity.
摘要翻译： 人类胚胎干细胞在含有分化的人细胞的培养物中形成胚状体。 胚胎体中的一些人细胞分化成心肌细胞。 在这里，参考在人类胚胎干细胞中衍生的心肌细胞在药物筛选方案中用于心脏毒性的机制来描述这些心肌细胞的生物和电学特性。
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公开(公告)号：US08956867B2
公开(公告)日：2015-02-17
申请号：US12615044
申请日：2009-11-09
CPC分类号： C07K16/18 , C12N5/0603 , C12N2533/90
摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.
摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。
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3.发明申请CARDIAC DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS UNDER DEFINED CONDITIONS USING MATRIX OVERLAY METHODS 有权使用矩阵覆盖方法定义的条件下人类间质干细胞的心脏分化公开(公告)号：US20110142935A1
公开(公告)日：2011-06-16
申请号：US12946019
申请日：2010-11-15
申请人： Timothy J. Kamp , Sean Palecek , Jianhua Zhang , Samira Azarin , Xiaojun Lian
发明人： Timothy J. Kamp , Sean Palecek , Jianhua Zhang , Samira Azarin , Xiaojun Lian
CPC分类号： C12N5/0657 , C12N2501/155 , C12N2501/16 , C12N2501/727 , C12N2506/02 , C12N2506/45
摘要： Methods for culturing the pluripotent stem cells to undergo epithelial-to-mesenchymal transition and for generating high-yield, high-purity cardiomyocyte cultures from pluripotent stem cells are described. Pluripotent stem cells are cultured on a support with an overlaid matrix and, optionally, exposed to one or more factors to induce epithelial-to-mesenchymal transition and cardiogenesis.
摘要翻译： 描述了培养多能干细胞进行上皮 - 间质转化和从多能干细胞产生高产量，高纯度心肌细胞培养物的方法。 多能干细胞在具有重叠基质的载体上培养，并且任选地暴露于一种或多种因子以诱导上皮 - 间质转化和心脏发生。
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4.发明授权Cardiac differentiation of human pluripotent stem cells under defined conditions using matrix overlay methods 有权在定义的条件下使用矩阵覆盖方法对人多能干细胞进行心脏分化公开(公告)号：US09068167B2
公开(公告)日：2015-06-30
申请号：US12946019
申请日：2010-11-15
申请人： Timothy J. Kamp , Sean Palecek , Jianhua Zhang , Samira Azarin , Xiaojun Lian
发明人： Timothy J. Kamp , Sean Palecek , Jianhua Zhang , Samira Azarin , Xiaojun Lian
CPC分类号： C12N5/0657 , C12N2501/155 , C12N2501/16 , C12N2501/727 , C12N2506/02 , C12N2506/45
摘要： Methods for culturing the pluripotent stem cells to undergo epithelial-to-mesenchymal transition and for generating high-yield, high-purity cardiomyocyte cultures from pluripotent stem cells are described. Pluripotent stem cells are cultured on a support with an overlaid matrix and, optionally, exposed to one or more factors to induce epithelial-to-mesenchymal transition and cardiogenesis.
摘要翻译： 描述了培养多能干细胞进行上皮 - 间质转化和从多能干细胞产生高产量，高纯度心肌细胞培养物的方法。 多能干细胞在具有重叠基质的载体上培养，并且任选地暴露于一种或多种因子以诱导上皮 - 间质转化和心脏发生。
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公开(公告)号：US20100197013A1
公开(公告)日：2010-08-05
申请号：US12615044
申请日：2009-11-09
CPC分类号： C07K16/18 , C12N5/0603 , C12N2533/90
摘要： A three-dimensional microwell system that supports long term pluripotent cell culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured pluripotent cells remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured pluripotent cells maintain pluripotency, differentiating to each of the three embryonic germ layers. Pluripotent cell aggregates released from microwells can be passaged for undifferentiated replication or differentiated to monodisperse EBs. The ability to constrain pluripotent cell growth in three dimensions advantageously provides for more efficient, reproducible culture of undifferentiated cells, high-throughput screening, and the ability to direct pluripotent cell differentiation by generating monodisperse EBs of a desired size and shape. Cardiomyocyte-rich EBs are obtained from pluripotent cells cultured in microwells of defined size and shape.
摘要翻译： 描述了支持长期多能细胞培养和形成均质胚状体（EB）的三维微孔系统。 微培养的多能细胞在培养物中保持存活和未分化数周，并在传播至经Matrigel®涂覆的组织培养处理的聚苯乙烯培养皿时保持未分化的复制。 微孔培养的多能细胞保持多能性，与三个胚胎胚层分化。 可以将从微孔释放的多能细胞聚集体传代为未分化复制或分化为单分散EB。 在三维中约束多能细胞生长的能力有利地提供了更有效，可重复的未分化细胞的培养，高通量筛选，以及通过产生所需大小和形状的单分散EB来引导多能细胞分化的能力。 富含心肌细胞的EB是从在规定尺寸和形状的微孔中培养的多能细胞获得的。
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