公开(公告)号：US20060292650A1

公开(公告)日：2006-12-28

申请号：US11438902

申请日：2006-05-23

申请人： James Braig ,  Kenneth Witte ,  Margaret Magarian ,  Jane Sheill ,  Bernhard Sterling

发明人： James Braig ,  Kenneth Witte ,  Margaret Magarian ,  Jane Sheill ,  Bernhard Sterling

IPC分类号： C12Q1/54 ,  C12M1/34

CPC分类号： C12Q1/26 ,  C12Q1/32 ,  C12Q1/52 ,  C12Q1/54

摘要： An apparatus is presented for estimating the concentration of an analyte using a combined enzyme-spectroscopic method. Examples are provided for the detection of glucose and lactate. A sample of biological fluid is mixed or contacted with an enzyme specific to the analyte of interest, and the reacting fluid is probed with an optical system at wavelengths that includes at least one wavelength that is sensitive to the analyte concentration and at least one wavelength that is not sensitive to the analyte concentration. The optical system measures properties, such as optical density, and relates the measurements to concentration through a calibration of the system. A method is also provided for analyzing the data obtained from optical measurements of reactions of enzymes with biological fluids. These technologies may be applied to continuous or periodic patient sampling systems or to test strip type devices.

摘要翻译： 提出了使用组合酶分光法估计分析物浓度的装置。 提供葡萄糖和乳酸盐检测的实例。 将生物流体样品与特定于目标分析物的酶混合或接触，并且反应流体用波长包括至少一种对分析物浓度敏感的波长和至少一种波长的光学系统进行探测 对分析物浓度不敏感。 光学系统测量诸如光密度的特性，并通过校准系统将测量值与浓度相关联。 还提供了一种用于分析从酶与生物流体的反应的光学测量获得的数据的方法。 这些技术可以应用于连续或周期性的患者采样系统或测试条型设备。