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公开(公告)号：US20170327767A1
公开(公告)日：2017-11-16
申请号：US15661673
申请日：2017-07-27
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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公开(公告)号：US20180087004A1
公开(公告)日：2018-03-29
申请号：US15642223
申请日：2017-07-05
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
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公开(公告)号：US20150018571A1
公开(公告)日：2015-01-15
申请号：US14332202
申请日：2014-07-15
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C11B3/00
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
摘要翻译： 本发明一般涉及重组脂肪酸合成领域，特别是在转基因植物中。 该应用描述了涉及脂肪酸合成的基因，并提供了用于操作植物油脂肪酸组成的方法和载体。 特别地，本发明提供了用于实现将参与脂肪酸合成的多个异源基因整合到植物基因组中的结构，使得所得植物产生改变水平的多不饱和脂肪酸。 还描述了通过共同表达植物储存器官内的沉默抑制基因来增强脂肪酸生物合成酶的表达的方法。
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公开(公告)号：US20180010141A1
公开(公告)日：2018-01-11
申请号：US15661697
申请日：2017-07-27
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. In particular, the present invention provides ω3 destaurases, Δ5 elongases and Δ6 desaturases with novel activities. Also provided are methods and DNA constructs for transiently and/or stably transforming cells, particularly plant cells, with multiple genes.
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公开(公告)号：US20180002633A1
公开(公告)日：2018-01-04
申请号：US15644635
申请日：2017-07-07
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
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公开(公告)号：US08809559B2
公开(公告)日：2014-08-19
申请号：US13129940
申请日：2009-11-17
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Maged Peter Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Zhou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C11B1/00
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
摘要翻译： 本发明一般涉及重组脂肪酸合成领域，特别是在转基因植物中。 该应用描述了涉及脂肪酸合成的基因，并提供了用于操作植物油脂肪酸组成的方法和载体。 特别地，本发明提供了用于实现将参与脂肪酸合成的多个异源基因整合到植物基因组中的结构，使得所得植物产生改变水平的多不饱和脂肪酸。 还描述了通过共同表达植物储存器官内的沉默抑制基因来增强脂肪酸生物合成酶的表达的方法。
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公开(公告)号：US20120016144A1
公开(公告)日：2012-01-19
申请号：US13129940
申请日：2009-11-17
申请人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Peter Maged Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Ziiou , Surinder Pal Singh , Craig Christopher Wood
发明人： James Robertson Petrie , Anne Maree Mackenzie , Qing Liu , Pushkar Shrestha , Peter David Nichols , Susan Irene Ellis Blackburn , Peter Maged Mansour , Stanley Suresh Robert , Dion Matthew Frederick Frampton , Xue-Rong Ziiou , Surinder Pal Singh , Craig Christopher Wood
IPC分类号： C11B1/10
CPC分类号： C11B3/006 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N9/93 , C12N15/52 , C12N15/8202 , C12N15/8205 , C12N15/8216 , C12N15/8218 , C12N15/8234 , C12N15/8247 , C12N15/8257 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12Y114/00 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates generally to the field of recombinant fatty acid synthesis, particularly in transgenic plants. The application describes genes involved in fatty acid synthesis and provides methods and vectors for the manipulation of fatty acid composition of plant oils. In particular, the invention provides constructs for achieving the integration of multiple heterologous genes involved in fatty acid synthesis into the plant genome, such that the resulting plants produce altered levels of polyunsaturated fatty acids. Also described are methods for enhancing the expression of fatty acid biosynthesis enzymes by co-expressing a silencing suppressor within the plant storage organ.
摘要翻译： 本发明一般涉及重组脂肪酸合成领域，特别是在转基因植物中。 该应用描述了涉及脂肪酸合成的基因，并提供了用于操作植物油脂肪酸组成的方法和载体。 特别地，本发明提供了用于实现将参与脂肪酸合成的多个异源基因整合到植物基因组中的结构，使得所得植物产生改变水平的多不饱和脂肪酸。 还描述了通过共同表达植物储存器官内的沉默抑制基因来增强脂肪酸生物合成酶的表达的方法。
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