公开(公告)号：US08569077B2

公开(公告)日：2013-10-29

申请号：US12467932

申请日：2009-05-18

申请人： Tyco M. Scholtens ,  Frederik Schreuder ,  Jan Greve ,  Arjan G. J. Tibbe ,  Leon W. M. M. Terstappen

发明人： Tyco M. Scholtens ,  Frederik Schreuder ,  Jan Greve ,  Arjan G. J. Tibbe ,  Leon W. M. M. Terstappen

IPC分类号： G01N33/553

CPC分类号： G01N33/54326

摘要： A method for removing excess unbound ferrofluid and imaging immunomagnetically enriched circulating tumor cells is provided. A vessels having a preformed grooves in the viewing surface is optimally designed for cell alignment and imaging. After separating the unbound particles by centrifugation, an externally-applied force is applied to transport magnetically responsive particle-CTC complex toward the transparent collection wall. The grooved inner surface of the viewing face of the chamber provide uniform distribution of the particles for easy imaging. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques as in quantitative analysis of CTC in disease.

摘要翻译： 提供了一种去除过量未结合的铁磁流体和成像免疫磁性富集的循环肿瘤细胞的方法。 在观察表面中具有预成形凹槽的容器被最佳设计用于细胞对准和成像。 通过离心分离未结合的颗粒后，施加外部施加的力以将磁响应颗粒-CTC复合物输送到透明收集壁。 室的观察面的带槽的内表面提供颗粒的均匀分布以便于成像。 本发明还可用于与自动细胞计数技术结合进行定量分析和样品制备，如在疾病中CTC的定量分析中。

公开(公告)号：US20090311734A1

公开(公告)日：2009-12-17

申请号：US12300344

申请日：2007-05-03

申请人： Jan Greve ,  Frederik Schreuder

发明人： Jan Greve ,  Frederik Schreuder

IPC分类号： C12Q1/02 ,  G01N33/68 ,  G01N33/48 ,  G01N21/64 ,  C12M1/34

CPC分类号： G01N33/54326 ,  Y10T436/143333

摘要： Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Time Delay Integration imaging (TDI). Incorporation of TDI technology into image cytometry analysis, like CellTracks®, makes it possible to image moving objects with very high sensitivity and signal-to-noise ratios. Implementation of TDI camera technology with dual excitation and multispectral imaging of enriched rare cells provides a rapid system for detection, enumeration, differentiation and characterization of imaged rare cells on the basis of size, morphology and immunophenotype.

摘要翻译： 公开了用于自动收集和图像分析的装置和方法，其能够识别或分类对准或沉积在表面上的微观物体。 这些物体，例如。 可检测标记的罕见靶细胞是磁性或非磁性固定的并经受时间延迟积分成像（TDI）。 将TDI技术结合到图像细胞分析中，像CellTracks（R），使得可以以非常高的灵敏度和信噪比来对移动物体进行成像。 基于大小，形态和免疫表型，实现了具有双重激发和多光谱成像的富集稀有细胞的TDI相机技术，提供了快速检测，计数，分化和表征成像稀有细胞的系统。

公开(公告)号：US20090286264A1

公开(公告)日：2009-11-19

申请号：US12467932

申请日：2009-05-18

申请人： Tyco M. Scholtens ,  Frederik Schreuder ,  Jan Greve ,  Arhjan G.J. Tibbe ,  Leon W.M.M. Terstappen

发明人： Tyco M. Scholtens ,  Frederik Schreuder ,  Jan Greve ,  Arhjan G.J. Tibbe ,  Leon W.M.M. Terstappen

IPC分类号： G01N33/53

CPC分类号： G01N33/54326

摘要： A method for removing excess unbound ferrofluid and imaging immunomagnetically enriched circulating tumor cells is provided. A vessels having a preformed grooves in the viewing surface is optimally designed for cell alignment and imaging. After separating the unbound particles by centrifugation, an externally-applied force is applied to transport magnetically responsive particle-CTC complex toward the transparent collection wall. The grooved inner surface of the viewing face of the chamber provide uniform distribution of the particles for easy imaging. The invention is also useful in conducting quantitative analysis and sample preparation in conjunction with automated cell enumeration techniques as in quantitative analysis of CTC in disease.

摘要翻译： 提供了一种去除过量未结合的铁磁流体和成像免疫磁性富集的循环肿瘤细胞的方法。 在观察表面中具有预成形凹槽的容器被最佳设计用于细胞对准和成像。 通过离心分离未结合的颗粒后，施加外部施加的力以将磁响应颗粒-CTC复合物输送到透明收集壁。 室的观察面的带槽的内表面提供颗粒的均匀分布以便于成像。 本发明还可用于与自动细胞计数技术结合进行定量分析和样品制备，如在疾病中CTC的定量分析中。