Plastid Transformation System to Prevent the Intramolecular Recombination of Transgene
    2.
    发明申请
    Plastid Transformation System to Prevent the Intramolecular Recombination of Transgene 失效
    质体转化体系,以防止转基因的分子内重组

    公开(公告)号:US20080301834A1

    公开(公告)日:2008-12-04

    申请号:US12159595

    申请日:2006-10-25

    IPC分类号: C12N15/82 A01H5/00

    CPC分类号: C12N15/8214

    摘要: The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.

    摘要翻译: 本发明涉及能够防止插入到质体基因组中的异源基因的第二重组事件的质体转化系统。 更具体地,本发明涉及携带启动子的质体转化载体和源自烟草以外的生物体的终止子。 本发明的用于质体转化的重组表达载体能够以与产生烟草来源的启动子/终止子对的常规载体水平相当的量产生外源蛋白质。 同时,它能够防止质体内的第二次重组事件。 因此,本发明的载体在生产转基因植物中是非常有用的,因为它可以有效地引入异源基因并支持正常转化和异源基因表达。

    Plastid transformation system to prevent the intramolecular recombination of transgene
    3.
    发明授权
    Plastid transformation system to prevent the intramolecular recombination of transgene 失效
    质体转化系统,以防止转基因的分子内重组

    公开(公告)号:US07888562B2

    公开(公告)日:2011-02-15

    申请号:US12159595

    申请日:2006-10-25

    IPC分类号: C12N15/82 A01H5/00

    CPC分类号: C12N15/8214

    摘要: The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.

    摘要翻译: 本发明涉及能够防止插入到质体基因组中的异源基因的第二重组事件的质体转化系统。 更具体地,本发明涉及携带启动子的质体转化载体和源自烟草以外的生物体的终止子。 本发明的用于质体转化的重组表达载体能够以与产生烟草来源的启动子/终止子对的常规载体水平相当的量产生外源蛋白质。 同时,它能够防止质体内的第二次重组事件。 因此,本发明的载体在生产转基因植物中是非常有用的,因为它可以有效地引入异源基因并支持正常转化和异源基因表达。